2gj0: Difference between revisions

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[[Image:2gj0.gif|left|200px]]
{{Seed}}
[[Image:2gj0.png|left|200px]]


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{{STRUCTURE_2gj0|  PDB=2gj0  |  SCENE=  }}  
{{STRUCTURE_2gj0|  PDB=2gj0  |  SCENE=  }}  


'''Cycloviolacin O14'''
===Cycloviolacin O14===




==Overview==
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Cyclotides are a fascinating family of plant-derived peptides characterized by their head-to-tail cyclized backbone and knotted arrangement of three disulfide bonds. This conserved structural architecture, termed the CCK (cyclic cystine knot), is responsible for their exceptional resistance to thermal, chemical and enzymatic degradation. Cyclotides have a variety of biological activities, but their insecticidal activities suggest that their primary function is in plant defence. In the present study, we determined the cyclotide content of the sweet violet Viola odorata, a member of the Violaceae family. We identified 30 cyclotides from the aerial parts and roots of this plant, 13 of which are novel sequences. The new sequences provide information about the natural diversity of cyclotides and the role of particular residues in defining structure and function. As many of the biological activities of cyclotides appear to be associated with membrane interactions, we used haemolytic activity as a marker of bioactivity for a selection of the new cyclotides. The new cyclotides were tested for their ability to resist proteolysis by a range of enzymes and, in common with other cyclotides, were completely resistant to trypsin, pepsin and thermolysin. The results show that while biological activity varies with the sequence, the proteolytic stability of the framework does not, and appears to be an inherent feature of the cyclotide framework. The structure of one of the new cyclotides, cycloviolacin O14, was determined and shown to contain the CCK motif. This study confirms that cyclotides may be regarded as a natural combinatorial template that displays a variety of peptide epitopes most likely targeted to a range of plant pests and pathogens.
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{{ABSTRACT_PUBMED_16872274}}


==About this Structure==
==About this Structure==
2GJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Viola_odorata Viola odorata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJ0 OCA].  
2GJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Viola_odorata Viola odorata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJ0 OCA].  


==Reference==
==Reference==
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[[Category: Cyclotide]]
[[Category: Cyclotide]]
[[Category: Plant protein]]
[[Category: Plant protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:15:10 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:14:45 2008''

Revision as of 21:14, 28 July 2008

File:2gj0.png

Template:STRUCTURE 2gj0

Cycloviolacin O14

Template:ABSTRACT PUBMED 16872274

About this Structure

2GJ0 is a Single protein structure of sequence from Viola odorata. Full experimental information is available from OCA.

Reference

A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability., Ireland DC, Colgrave ML, Craik DJ, Biochem J. 2006 Nov 15;400(1):1-12. PMID:16872274

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