3b8s: Difference between revisions

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[[Image:3b8s.jpg|left|200px]]
{{Seed}}
[[Image:3b8s.png|left|200px]]


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{{STRUCTURE_3b8s|  PDB=3b8s  |  SCENE=  }}  
{{STRUCTURE_3b8s|  PDB=3b8s  |  SCENE=  }}  


'''Crystal structure of wild-type chitinase A from Vibrio harveyi'''
===Crystal structure of wild-type chitinase A from Vibrio harveyi===




==Overview==
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This research describes four X-ray structures of Vibrio harveyi chitinase A and its catalytically inactive mutant (E315M) in the presence and absence of substrates. The overall structure of chitinase A is that of a typical family-18 glycosyl hydrolase comprising three distinct domains: (i) the amino-terminal chitin-binding domain; (ii) the main catalytic (alpha/beta)(8) TIM-barrel domain; and (iii) the small (alpha+beta) insertion domain. The catalytic cleft of chitinase A has a long, deep groove, which contains six chitooligosaccharide ring-binding subsites (-4)(-3)(-2)(-1)(+1)(+2). The binding cleft of the ligand-free E315M is partially blocked by the C-terminal (His)(6)-tag. Structures of E315M-chitooligosaccharide complexes display a linear conformation of pentaNAG, but a bent conformation of hexaNAG. Analysis of the final 2F(o)-F(c) omit map of E315M-NAG6 reveals the existence of the linear conformation of the hexaNAG at a lower occupancy with respect to the bent conformation. These crystallographic data provide evidence that the interacting sugars undergo conformational changes prior to hydrolysis by the wild-type enzyme.
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{{ABSTRACT_PUBMED_18467126}}


==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Tim-barrel]]
[[Category: Tim-barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:20:06 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:06:02 2008''

Revision as of 17:06, 28 July 2008

File:3b8s.png

Template:STRUCTURE 3b8s

Crystal structure of wild-type chitinase A from Vibrio harveyi

Template:ABSTRACT PUBMED 18467126

About this Structure

3B8S is a Single protein structure of sequence from Vibrio harveyi. Full crystallographic information is available from OCA.

Reference

Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism., Songsiriritthigul C, Pantoom S, Aguda AH, Robinson RC, Suginta W, J Struct Biol. 2008 Jun;162(3):491-9. Epub 2008 Mar 26. PMID:18467126

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