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| [[Image:3c48.jpg|left|200px]] | | {{Seed}} |
| | [[Image:3c48.png|left|200px]] |
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| {{STRUCTURE_3c48| PDB=3c48 | SCENE= }} | | {{STRUCTURE_3c48| PDB=3c48 | SCENE= }} |
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| '''Structure of the retaining glycosyltransferase MshA: The first step in mycothiol biosynthesis. Organism: Corynebacterium glutamicum- APO (OPEN) structure.'''
| | ===Structure of the retaining glycosyltransferase MshA: The first step in mycothiol biosynthesis. Organism: Corynebacterium glutamicum- APO (OPEN) structure.=== |
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| ==Overview==
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| The glycosyltransferase termed MshA catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to 1-l-myo-inositol-1-phosphate in the first committed step of mycothiol biosynthesis. The structure of MshA from Corynebacterium glutamicum was determined both in the absence of substrates and in a complex with UDP and 1-l-myo-inositol-1-phosphate. MshA belongs to the GT-B structural family whose members have a two-domain structure with both domains exhibiting a Rossman-type fold. Binding of the donor sugar to the C-terminal domain produces a 97 degrees rotational reorientation of the N-terminal domain relative to the C-terminal domain, clamping down on UDP and generating the binding site for 1-l-myo-inositol-1-phosphate. The structure highlights the residues important in binding of UDP-N-acetylglucosamine and 1-l-myo-inositol-1-phosphate. Molecular models of the ternary complex suggest a mechanism in which the beta-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attack of the 3-hydroxyl group of 1-l-myo-inositol-1-phosphate while at the same time promoting the cleavage of the sugar nucleotide bond.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18390549}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18390549 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18390549}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Retaining glycosyltransferase]] | | [[Category: Retaining glycosyltransferase]] |
| [[Category: Substrate assisted catalysis]] | | [[Category: Substrate assisted catalysis]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:21:26 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:29:39 2008'' |
