1cld: Difference between revisions

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[[Image:1cld.jpg|left|200px]]<br /><applet load="1cld" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1cld.jpg|left|200px]]<br /><applet load="1cld" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1cld" />

'''DNA-BINDING PROTEIN'''<br />

==Overview==

The Zn2Cys6 DNA-binding domain has been identified by sequence homology in, approximately forty fungal proteins, including the K. lactis LAC9, transcriptional activator. Using 1H NMR spectroscopy, we have determined, the solution structure of a cadmium-substituted form of the LAC9, DNA-binding domain. We have complemented this approach by applying a, series of 113Cd-1H NMR experiments, including several novel, heteroTOCSY-based techniques. The DNA-binding domain forms a core of two, alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds, stabilizing the cluster. Comparison with other Zn2Cys6 domain structures, provides insight into the common structural elements used in metal, coordination and DNA binding.

The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.

==About this Structure==

1CLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA].

1CLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA].

==Reference==

Line 13:

[[Category: Kluyveromyces lactis]]

[[Category: Single protein]]

[[Category: Coleman, J.E.]]

[[Category: Coleman, J E.]]

[[Category: Gardner, K.H.]]

[[Category: Gardner, K H.]]

[[Category: CD]]

[[Category: zinc-binding domain]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:16 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1cld.jpg|left|200px]]<br /><applet load="1cld" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cld.jpg|left|200px]]<br /><applet load="1cld" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1cld" />
 '''DNA-BINDING PROTEIN'''<br />
 ==Overview==
-The Zn2Cys6 DNA-binding domain has been identified by sequence homology in, approximately forty fungal proteins, including the K. lactis LAC9, transcriptional activator. Using 1H NMR spectroscopy, we have determined, the solution structure of a cadmium-substituted form of the LAC9, DNA-binding domain. We have complemented this approach by applying a, series of 113Cd-1H NMR experiments, including several novel, heteroTOCSY-based techniques. The DNA-binding domain forms a core of two, alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds, stabilizing the cluster. Comparison with other Zn2Cys6 domain structures, provides insight into the common structural elements used in metal, coordination and DNA binding.
+The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.
 ==About this Structure==
-CLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA].
+CLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Kluyveromyces lactis]]
 [[Category: Single protein]]
-[[Category: Coleman, J.E.]]
+[[Category: Coleman, J E.]]
-[[Category: Gardner, K.H.]]
+[[Category: Gardner, K H.]]
 [[Category: CD]]
 [[Category: zinc-binding domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:33:35 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:16 2008''