1cyq: Difference between revisions

Line 1:

[[Image:1cyq.gif|left|200px]]<br /><applet load="1cyq" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1cyq.gif|left|200px]]<br /><applet load="1cyq" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1cyq, resolution 1.93Å" />

'''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX'''<br />

==Overview==

A novel mechanism of DNA endonucleolytic cleavage has been visualized for, the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate, complex and comparing it to the previously visualized product complex., This enzyme employs a unique single metal mechanism. A magnesium ion is, coordinated by an asparagine residue and two DNA oxygen atoms and, stabilizes the phosphoanion transition state and the 3'oxygen leaving, group. A hydrolytic water molecule is activated by a histidine residue for, an in-line attack on the scissile phosphate. A strained, enzyme-substrate-metal complex is formed before cleavage, then relaxed, during the reaction.

A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.

==About this Structure==

1CYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA].

1CYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Chevalier, B.]]

[[Category: Flick, K.E.]]

[[Category: Flick, K E.]]

[[Category: Galburt, E.A.]]

[[Category: Galburt, E A.]]

[[Category: Jurica, M.S.]]

[[Category: Jurica, M S.]]

[[Category: Stoddard, B.L.]]

[[Category: Stoddard, B L.]]

[[Category: MG]]

[[Category: ZN]]

Line 25:

[[Category: protein-dna complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 12:51:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:11:04 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1cyq.gif|left|200px]]<br /><applet load="1cyq" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cyq.gif|left|200px]]<br /><applet load="1cyq" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1cyq, resolution 1.93&Aring;" />
 '''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX'''<br />
 ==Overview==
-A novel mechanism of DNA endonucleolytic cleavage has been visualized for, the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate, complex and comparing it to the previously visualized product complex., This enzyme employs a unique single metal mechanism. A magnesium ion is, coordinated by an asparagine residue and two DNA oxygen atoms and, stabilizes the phosphoanion transition state and the 3'oxygen leaving, group. A hydrolytic water molecule is activated by a histidine residue for, an in-line attack on the scissile phosphate. A strained, enzyme-substrate-metal complex is formed before cleavage, then relaxed, during the reaction.
+A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.
 ==About this Structure==
-CYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with ZN and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA].
+CYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Chevalier, B.]]
-[[Category: Flick, K.E.]]
+[[Category: Flick, K E.]]
-[[Category: Galburt, E.A.]]
+[[Category: Galburt, E A.]]
-[[Category: Jurica, M.S.]]
+[[Category: Jurica, M S.]]
-[[Category: Stoddard, B.L.]]
+[[Category: Stoddard, B L.]]
 [[Category: MG]]
 [[Category: ZN]]
@@ Line 25: / Line 25: @@
 [[Category: protein-dna complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:51:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:11:04 2008''