1d3u: Difference between revisions

Line 1:

[[Image:1d3u.gif|left|200px]]<br /><applet load="1d3u" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1d3u.gif|left|200px]]<br /><applet load="1d3u" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1d3u, resolution 2.4Å" />

'''TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI'''<br />

==Overview==

Recently the definition of the metazoan RNA polymerase II and archaeal, core promoters has been expanded to include a region immediately upstream, of the TATA box called the B recognition element (BRE), so named because, eukaryal transcription factor TFIIB and its archaeal orthologue TFB, interact with the element in a sequence-specific manner. Here we present, the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB, (TFB(c)) in a complex with an extended TATA-box-containing promoter that, provides a detailed picture of the stereospecific interactions between the, BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of, TFB(c). This interaction is important in determining the level of basal, transcription and explicitly defines the direction of transcription.

Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription.

==About this Structure==

1D3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1D3U OCA].

1D3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3U OCA].

==Reference==

Line 15:

[[Category: Korkhin, Y.]]

[[Category: Littlefield, O.]]

[[Category: Sigler, P.B.]]

[[Category: Sigler, P B.]]

[[Category: hyperthermophile]]

[[Category: protein-dna complex]]

[[Category: transcription]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 12:59:~~34 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1d3u.gif|left|200px]]<br /><applet load="1d3u" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d3u.gif|left|200px]]<br /><applet load="1d3u" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d3u, resolution 2.4&Aring;" />
 '''TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI'''<br />
 ==Overview==
-Recently the definition of the metazoan RNA polymerase II and archaeal, core promoters has been expanded to include a region immediately upstream, of the TATA box called the B recognition element (BRE), so named because, eukaryal transcription factor TFIIB and its archaeal orthologue TFB, interact with the element in a sequence-specific manner. Here we present, the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB, (TFB(c)) in a complex with an extended TATA-box-containing promoter that, provides a detailed picture of the stereospecific interactions between the, BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of, TFB(c). This interaction is important in determining the level of basal, transcription and explicitly defines the direction of transcription.
+Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription.
 ==About this Structure==
-D3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D3U OCA].
+D3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_woesei Pyrococcus woesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3U OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Korkhin, Y.]]
 [[Category: Littlefield, O.]]
-[[Category: Sigler, P.B.]]
+[[Category: Sigler, P B.]]
 [[Category: hyperthermophile]]
 [[Category: protein-dna complex]]
 [[Category: transcription]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:59:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:40 2008''