1d6a: Difference between revisions
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New page: left|200px<br /><applet load="1d6a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d6a, resolution 2.1Å" /> '''STRUCTURE OF POKEWEED... |
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[[Image:1d6a.gif|left|200px]]<br /><applet load="1d6a" size=" | [[Image:1d6a.gif|left|200px]]<br /><applet load="1d6a" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1d6a, resolution 2.1Å" /> | caption="1d6a, resolution 2.1Å" /> | ||
'''STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH GUANINE'''<br /> | '''STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH GUANINE'''<br /> | ||
==Overview== | ==Overview== | ||
Pokeweed antiviral protein (PAP) is a ribosome-inactivating protein (RIP), which enzymatically removes a single adenine base from a conserved, surface exposed loop sequence of ribosomal rRNA. We now present | Pokeweed antiviral protein (PAP) is a ribosome-inactivating protein (RIP), which enzymatically removes a single adenine base from a conserved, surface exposed loop sequence of ribosomal rRNA. We now present unprecedented experimental evidence that PAP can release not only adenine but guanine as well from Escherichia coli rRNA, albeit at a rate 20 times slower than for adenine. We also report X-ray structure analysis and supporting modeling studies for the interactions of PAP with guanine. Our modeling studies indicated that PAP can accommodate a guanine base in the active site pocket without large conformational changes. This prediction was experimentally confirmed, since a guanine base was visible in the active site pocket of the crystal structure of the PAP-guanine complex. | ||
==About this Structure== | ==About this Structure== | ||
1D6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with GUN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http:// | 1D6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana] with <scene name='pdbligand=GUN:'>GUN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6A OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: rRNA N-glycosylase]] | [[Category: rRNA N-glycosylase]] | ||
[[Category: Kurinov, I | [[Category: Kurinov, I V.]] | ||
[[Category: Rajamohan, F.]] | [[Category: Rajamohan, F.]] | ||
[[Category: Uckun, F | [[Category: Uckun, F M.]] | ||
[[Category: Venkatachalam, T | [[Category: Venkatachalam, T K.]] | ||
[[Category: GUN]] | [[Category: GUN]] | ||
[[Category: pokeweed antiviral protein]] | [[Category: pokeweed antiviral protein]] | ||
[[Category: ribosome inactivating protein]] | [[Category: ribosome inactivating protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:23 2008'' | ||
