1dbr: Difference between revisions

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[[Image:1dbr.jpg|left|200px]]<br /><applet load="1dbr" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1dbr.jpg|left|200px]]<br /><applet load="1dbr" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1dbr, resolution 2.4Å" />

'''HYPOXANTHINE GUANINE XANTHINE'''<br />

==Overview==

Crystal structures of substrate-free and XMP-soaked, hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) of the, opportunistic pathogen Toxoplasma gondii have been determined to 2.4 and, 2.9 A resolution, respectively. HGXPRTase displays the conserved PRTase, fold. In the structure of the enzyme bound to its product, a long flexible, loop (residues 115-126) is located away from the active site. Comparison, to the substrate-free structure reveals a striking relocation of the loop, which is poised to cover the catalytic pocket, thus providing a mechanism, by which the HG(X)PRTases shield their oxocarbonium transition states from, nucleophilic attack by the bulk solvent. The conserved Ser 117-Tyr 118, dipeptide within the loop is brought to the active site, completing the, ensemble of catalytic residues.

Crystal structures of substrate-free and XMP-soaked hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) of the opportunistic pathogen Toxoplasma gondii have been determined to 2.4 and 2.9 A resolution, respectively. HGXPRTase displays the conserved PRTase fold. In the structure of the enzyme bound to its product, a long flexible loop (residues 115-126) is located away from the active site. Comparison to the substrate-free structure reveals a striking relocation of the loop, which is poised to cover the catalytic pocket, thus providing a mechanism by which the HG(X)PRTases shield their oxocarbonium transition states from nucleophilic attack by the bulk solvent. The conserved Ser 117-Tyr 118 dipeptide within the loop is brought to the active site, completing the ensemble of catalytic residues.

==About this Structure==

1DBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DBR OCA].

1DBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBR OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Toxoplasma gondii]]

[[Category: Brennan, R.G.]]

[[Category: Brennan, R G.]]

[[Category: Carter, D.]]

[[Category: Roos, D.]]

[[Category: Schumacher, M.A.]]

[[Category: Schumacher, M A.]]

[[Category: Ullman, B.]]

[[Category: MG]]

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[[Category: transferase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 13~~:08:~~41 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:58 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1dbr.jpg|left|200px]]<br /><applet load="1dbr" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dbr.jpg|left|200px]]<br /><applet load="1dbr" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1dbr, resolution 2.4&Aring;" />
 '''HYPOXANTHINE GUANINE XANTHINE'''<br />
 ==Overview==
-Crystal structures of substrate-free and XMP-soaked, hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) of the, opportunistic pathogen Toxoplasma gondii have been determined to 2.4 and, 2.9 A resolution, respectively. HGXPRTase displays the conserved PRTase, fold. In the structure of the enzyme bound to its product, a long flexible, loop (residues 115-126) is located away from the active site. Comparison, to the substrate-free structure reveals a striking relocation of the loop, which is poised to cover the catalytic pocket, thus providing a mechanism, by which the HG(X)PRTases shield their oxocarbonium transition states from, nucleophilic attack by the bulk solvent. The conserved Ser 117-Tyr 118, dipeptide within the loop is brought to the active site, completing the, ensemble of catalytic residues.
+Crystal structures of substrate-free and XMP-soaked hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) of the opportunistic pathogen Toxoplasma gondii have been determined to 2.4 and 2.9 A resolution, respectively. HGXPRTase displays the conserved PRTase fold. In the structure of the enzyme bound to its product, a long flexible loop (residues 115-126) is located away from the active site. Comparison to the substrate-free structure reveals a striking relocation of the loop, which is poised to cover the catalytic pocket, thus providing a mechanism by which the HG(X)PRTases shield their oxocarbonium transition states from nucleophilic attack by the bulk solvent. The conserved Ser 117-Tyr 118 dipeptide within the loop is brought to the active site, completing the ensemble of catalytic residues.
 ==About this Structure==
-DBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DBR OCA].
+DBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBR OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Toxoplasma gondii]]
-[[Category: Brennan, R.G.]]
+[[Category: Brennan, R G.]]
 [[Category: Carter, D.]]
 [[Category: Roos, D.]]
-[[Category: Schumacher, M.A.]]
+[[Category: Schumacher, M A.]]
 [[Category: Ullman, B.]]
 [[Category: MG]]
@@ Line 23: / Line 23: @@
 [[Category: transferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:08:41 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:58 2008''