1dch: Difference between revisions

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[[Image:1dch.gif|left|200px]]<br /><applet load="1dch" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1dch.gif|left|200px]]<br /><applet load="1dch" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1dch, resolution 3.0Å" />

'''CRYSTAL STRUCTURE OF DCOH, A BIFUNCTIONAL, PROTEIN-BINDING TRANSCRIPTION COACTIVATOR'''<br />

==Overview==

DCoH, the dimerization cofactor of hepatocyte nuclear factor-1, stimulates, gene expression by associating with specific DNA binding proteins and also, catalyzes the dehydration of the biopterin cofactor of phenylalanine, hydroxylase. The x-ray crystal structure determined at 3 angstrom, resolution reveals that DCoH forms a tetramer containing two saddle-shaped, grooves that comprise likely macromolecule binding sites. Two equivalent, enzyme active sites flank each saddle, suggesting that there is a spatial, connection between the catalytic and binding activities. Structural, similarities between the DCoH fold and nucleic acid-binding proteins argue, that the saddle motif has evolved to bind diverse ligands or that DCoH, unexpectedly may bind nucleic acids.

DCoH, the dimerization cofactor of hepatocyte nuclear factor-1, stimulates gene expression by associating with specific DNA binding proteins and also catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase. The x-ray crystal structure determined at 3 angstrom resolution reveals that DCoH forms a tetramer containing two saddle-shaped grooves that comprise likely macromolecule binding sites. Two equivalent enzyme active sites flank each saddle, suggesting that there is a spatial connection between the catalytic and binding activities. Structural similarities between the DCoH fold and nucleic acid-binding proteins argue that the saddle motif has evolved to bind diverse ligands or that DCoH unexpectedly may bind nucleic acids.

==About this Structure==

1DCH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DCH OCA].

1DCH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCH OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Alber, T.]]

[[Category: Crabtree, G.R.]]

[[Category: Crabtree, G R.]]

[[Category: Cronk, J.D.]]

[[Category: Cronk, J D.]]

[[Category: Endrizzi, J.A.]]

[[Category: Endrizzi, J A.]]

[[Category: Wang, W.]]

[[Category: SO4]]

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[[Category: transregulator of homeodomain proteins]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:12 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1dch.gif|left|200px]]<br /><applet load="1dch" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dch.gif|left|200px]]<br /><applet load="1dch" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1dch, resolution 3.0&Aring;" />
 '''CRYSTAL STRUCTURE OF DCOH, A BIFUNCTIONAL, PROTEIN-BINDING TRANSCRIPTION COACTIVATOR'''<br />
 ==Overview==
-DCoH, the dimerization cofactor of hepatocyte nuclear factor-1, stimulates, gene expression by associating with specific DNA binding proteins and also, catalyzes the dehydration of the biopterin cofactor of phenylalanine, hydroxylase. The x-ray crystal structure determined at 3 angstrom, resolution reveals that DCoH forms a tetramer containing two saddle-shaped, grooves that comprise likely macromolecule binding sites. Two equivalent, enzyme active sites flank each saddle, suggesting that there is a spatial, connection between the catalytic and binding activities. Structural, similarities between the DCoH fold and nucleic acid-binding proteins argue, that the saddle motif has evolved to bind diverse ligands or that DCoH, unexpectedly may bind nucleic acids.
+DCoH, the dimerization cofactor of hepatocyte nuclear factor-1, stimulates gene expression by associating with specific DNA binding proteins and also catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase. The x-ray crystal structure determined at 3 angstrom resolution reveals that DCoH forms a tetramer containing two saddle-shaped grooves that comprise likely macromolecule binding sites. Two equivalent enzyme active sites flank each saddle, suggesting that there is a spatial connection between the catalytic and binding activities. Structural similarities between the DCoH fold and nucleic acid-binding proteins argue that the saddle motif has evolved to bind diverse ligands or that DCoH unexpectedly may bind nucleic acids.
 ==About this Structure==
-DCH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DCH OCA].
+DCH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCH OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Alber, T.]]
-[[Category: Crabtree, G.R.]]
+[[Category: Crabtree, G R.]]
-[[Category: Cronk, J.D.]]
+[[Category: Cronk, J D.]]
-[[Category: Endrizzi, J.A.]]
+[[Category: Endrizzi, J A.]]
 [[Category: Wang, W.]]
 [[Category: SO4]]
@@ Line 25: / Line 25: @@
 [[Category: transregulator of homeodomain proteins]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:10:11 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:12 2008''