1dfi: Difference between revisions
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New page: left|200px<br /><applet load="1dfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dfi, resolution 2.09Å" /> '''X-RAY STRUCTURE OF E... |
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[[Image:1dfi.jpg|left|200px]]<br /><applet load="1dfi" size=" | [[Image:1dfi.jpg|left|200px]]<br /><applet load="1dfi" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1dfi, resolution 2.09Å" /> | caption="1dfi, resolution 2.09Å" /> | ||
'''X-RAY STRUCTURE OF ESCHERICHIA COLI ENOYL REDUCTASE WITH BOUND NAD'''<br /> | '''X-RAY STRUCTURE OF ESCHERICHIA COLI ENOYL REDUCTASE WITH BOUND NAD'''<br /> | ||
==Overview== | ==Overview== | ||
Enoyl reductase (ENR), an enzyme involved in fatty acid biosynthesis, is | Enoyl reductase (ENR), an enzyme involved in fatty acid biosynthesis, is the target for antibacterial diazaborines and the front-line antituberculosis drug isoniazid. Analysis of the structures of complexes of Escherichia coli ENR with nicotinamide adenine dinucleotide and either thienodiazaborine or benzodiazaborine revealed the formation of a covalent bond between the 2' hydroxyl of the nicotinamide ribose and a boron atom in the drugs to generate a tight, noncovalently bound bisubstrate analog. This analysis has implications for the structure-based design of inhibitors of ENR, and similarities to other oxidoreductases suggest that mimicking this molecular linkage may have generic applications in other areas of medicinal chemistry. | ||
==About this Structure== | ==About this Structure== | ||
1DFI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] Full crystallographic information is available from [http:// | 1DFI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DFI OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Baldock, C.]] | [[Category: Baldock, C.]] | ||
[[Category: Rafferty, J | [[Category: Rafferty, J B.]] | ||
[[Category: Rice, D | [[Category: Rice, D W.]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
[[Category: lipid biosynthesis]] | [[Category: lipid biosynthesis]] | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:17 2008'' | ||
