1dhr: Difference between revisions
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New page: left|200px<br /><applet load="1dhr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dhr, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF ... |
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[[Image:1dhr.gif|left|200px]]<br /><applet load="1dhr" size=" | [[Image:1dhr.gif|left|200px]]<br /><applet load="1dhr" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1dhr, resolution 2.3Å" /> | caption="1dhr, resolution 2.3Å" /> | ||
'''CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE'''<br /> | '''CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of a binary complex of dihydropteridine reductase [DHPR; | The structure of a binary complex of dihydropteridine reductase [DHPR; NAD(P)H:6,7-dihydropteridine oxidoreductase, EC 1.6.99.7] with its cofactor, NADH, has been solved and refined to a final R factor of 15.4% by using 2.3 A diffraction data. DHPR is an alpha/beta protein with a Rossmann-type dinucleotide fold for NADH binding. Insertion of an extra threonine residue in the human enzyme is associated with severe symptoms of a variant form of phenylketonuria and maps to a tightly linked sequence of secondary-structural elements near the dimer interface. Dimerization is mediated by a four-helix bundle motif (two helices from each protomer) having an unusual right-handed twist. DHPR is structurally and mechanistically distinct from dihydrofolate reductase, appearing to more closely resemble certain nicotinamide dinucleotide-requiring flavin-dependent enzymes, such as glutathione reductase. | ||
==About this Structure== | ==About this Structure== | ||
1DHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] Full crystallographic information is available from [http:// | 1DHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DHR OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Matthews, D | [[Category: Matthews, D A.]] | ||
[[Category: Skinner, M | [[Category: Skinner, M M.]] | ||
[[Category: Varughese, K | [[Category: Varughese, K I.]] | ||
[[Category: Whiteley, J | [[Category: Whiteley, J M.]] | ||
[[Category: Xuong, N | [[Category: Xuong, N H.]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
[[Category: oxidoreductase(acting on nadh or nadph)]] | [[Category: oxidoreductase(acting on nadh or nadph)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:40 2008'' | ||
