1dj0: Difference between revisions
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New page: left|200px<br /><applet load="1dj0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dj0, resolution 1.50Å" /> '''THE CRYSTAL STRUCTUR... |
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[[Image:1dj0.jpg|left|200px]]<br /><applet load="1dj0" size=" | [[Image:1dj0.jpg|left|200px]]<br /><applet load="1dj0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1dj0, resolution 1.50Å" /> | caption="1dj0, resolution 1.50Å" /> | ||
'''THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION'''<br /> | '''THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
Pseudouridine synthases catalyze the isomerization of specific uridines to | Pseudouridine synthases catalyze the isomerization of specific uridines to pseudouridine in a variety of RNAs, yet the basis for recognition of the RNA sites or how they catalyze this reaction is unknown. The crystal structure of pseudouridine synthase I from Escherichia coli, which, for example, modifies positions 38, 39 and/or 40 in tRNA, reveals a dimeric protein that contains two positively charged, RNA-binding clefts along the surface of the protein. Each cleft contains a highly conserved aspartic acid located at its center. The structural domains have a topological similarity to those of other RNA-binding proteins, though the mode of interaction with tRNA appears to be unique. The structure suggests that a dimeric enzyme is required for binding transfer RNA and subsequent pseudouridine formation. | ||
==About this Structure== | ==About this Structure== | ||
1DJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http:// | 1DJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Pseudouridylate synthase]] | [[Category: Pseudouridylate synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Foster, P | [[Category: Foster, P G.]] | ||
[[Category: Huang, L.]] | [[Category: Huang, L.]] | ||
[[Category: Santi, D | [[Category: Santi, D V.]] | ||
[[Category: Stroud, R | [[Category: Stroud, R M.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: alpha/beta fold]] | [[Category: alpha/beta fold]] | ||
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[[Category: rna-modifying enzyme]] | [[Category: rna-modifying enzyme]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:11 2008'' | ||
