1dl0: Difference between revisions

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[[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1dl0" />

'''SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C'''<br />

==Overview==

We have isolated a family of insect-selective neurotoxins from the venom, of the Australian funnel-web spider that appear to be good candidates for, biopesticide engineering. These peptides, which we have named the, Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with, four disulfide bridges, and they show no homology to any sequences in the, protein/DNA databases. The three-dimensional structure of one of these, toxins reveals an extremely rare vicinal disulfide bridge that we, demonstrate to be critical for insecticidal activity. We propose that, J-ACTX comprises an ancestral protein fold that we refer to as the, disulfide-directed beta-hairpin.

We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.

==About this Structure==

1DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA].

1DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA].

==Reference==

Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10881200 10881200]

[[Category: Single protein]]

[[Category: King, G.F.]]

[[Category: King, G F.]]

[[Category: Wang, X.H.]]

[[Category: Wang, X H.]]

[[Category: atracotoxin]]

[[Category: cystine knot]]

Line 20:

[[Category: vicinal disulfide]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 13~~:22:~~41 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:43 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1dl0" />
 '''SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C'''<br />
 ==Overview==
-We have isolated a family of insect-selective neurotoxins from the venom, of the Australian funnel-web spider that appear to be good candidates for, biopesticide engineering. These peptides, which we have named the, Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with, four disulfide bridges, and they show no homology to any sequences in the, protein/DNA databases. The three-dimensional structure of one of these, toxins reveals an extremely rare vicinal disulfide bridge that we, demonstrate to be critical for insecticidal activity. We propose that, J-ACTX comprises an ancestral protein fold that we refer to as the, disulfide-directed beta-hairpin.
+We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.
 ==About this Structure==
-DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA].
+DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA].
 ==Reference==
 Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10881200 10881200]
 [[Category: Single protein]]
-[[Category: King, G.F.]]
+[[Category: King, G F.]]
-[[Category: Wang, X.H.]]
+[[Category: Wang, X H.]]
 [[Category: atracotoxin]]
 [[Category: cystine knot]]
@@ Line 20: / Line 20: @@
 [[Category: vicinal disulfide]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:22:41 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:43 2008''