1eej: Difference between revisions
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New page: left|200px<br /><applet load="1eej" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eej, resolution 1.90Å" /> '''CRYSTAL STRUCTURE OF... |
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[[Image:1eej.jpg|left|200px]]<br /><applet load="1eej" size=" | [[Image:1eej.jpg|left|200px]]<br /><applet load="1eej" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1eej, resolution 1.90Å" /> | caption="1eej, resolution 1.90Å" /> | ||
'''CRYSTAL STRUCTURE OF THE PROTEIN DISULFIDE BOND ISOMERASE, DSBC, FROM ESCHERICHIA COLI'''<br /> | '''CRYSTAL STRUCTURE OF THE PROTEIN DISULFIDE BOND ISOMERASE, DSBC, FROM ESCHERICHIA COLI'''<br /> | ||
==Overview== | ==Overview== | ||
DsbC is one of five Escherichia coli proteins required for disulfide bond | DsbC is one of five Escherichia coli proteins required for disulfide bond formation and is thought to function as a disulfide bond isomerase during oxidative protein folding in the periplasm. DsbC is a 2 x 23 kDa homodimer and has both protein disulfide isomerase and chaperone activity. We report the 1.9 A resolution crystal structure of oxidized DsbC where both Cys-X-X-Cys active sites form disulfide bonds. The molecule consists of separate thioredoxin-like domains joined via hinged linker helices to an N-terminal dimerization domain. The hinges allow relative movement of the active sites, and a broad uncharged cleft between them may be involved in peptide binding and DsbC foldase activities. | ||
==About this Structure== | ==About this Structure== | ||
1EEJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MES as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] Full crystallographic information is available from [http:// | 1EEJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EEJ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein disulfide-isomerase]] | [[Category: Protein disulfide-isomerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Baker, E | [[Category: Baker, E N.]] | ||
[[Category: Haebel, P | [[Category: Haebel, P W.]] | ||
[[Category: McCarthy, A | [[Category: McCarthy, A A.]] | ||
[[Category: Metcalf, P.]] | [[Category: Metcalf, P.]] | ||
[[Category: Rybin, V.]] | [[Category: Rybin, V.]] | ||
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[[Category: redox-active center]] | [[Category: redox-active center]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:57 2008'' | ||
