1ej0: Difference between revisions

Line 1:

[[Image:1ej0.jpg|left|200px]]<br /><applet load="1ej0" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ej0.jpg|left|200px]]<br /><applet load="1ej0" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ej0, resolution 1.50Å" />

'''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE'''<br />

==Overview==

Structural, biochemical, and genetic techniques were applied to, investigate the function of FtsJ, a recently identified heat shock, protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A, crystal structure of FtsJ in complex with its cofactor, S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The, molecular surface of FtsJ exposes a putative nucleic acid binding groove, composed of highly conserved, positively charged residues. Substrate, analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal, subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically, altered ribosome profile, a severe growth disadvantage, and a, temperature-sensitive phenotype. Our results reveal an unexpected link, between the heat shock response and RNA metabolism.

Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.

==About this Structure==

1EJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG and SAM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA].

1EJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Bardwell, J.C.A.]]

[[Category: Bardwell, J C.A.]]

[[Category: Bugl, H.]]

[[Category: Fauman, E.B.]]

[[Category: Fauman, E B.]]

[[Category: Jakob, U.]]

[[Category: Kushner, S.R.]]

[[Category: Kushner, S R.]]

[[Category: Saper, M.A.]]

[[Category: Saper, M A.]]

[[Category: Staker, B.L.]]

[[Category: Staker, B L.]]

[[Category: Zheng, F.]]

[[Category: HG]]

Line 30:

[[Category: methyltransferase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 14~~:02:~~23 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:15 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1ej0.jpg|left|200px]]<br /><applet load="1ej0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ej0.jpg|left|200px]]<br /><applet load="1ej0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ej0, resolution 1.50&Aring;" />
 '''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE'''<br />
 ==Overview==
-Structural, biochemical, and genetic techniques were applied to, investigate the function of FtsJ, a recently identified heat shock, protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A, crystal structure of FtsJ in complex with its cofactor, S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The, molecular surface of FtsJ exposes a putative nucleic acid binding groove, composed of highly conserved, positively charged residues. Substrate, analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal, subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically, altered ribosome profile, a severe growth disadvantage, and a, temperature-sensitive phenotype. Our results reveal an unexpected link, between the heat shock response and RNA metabolism.
+Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.
 ==About this Structure==
-EJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG and SAM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA].
+EJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Bardwell, J.C.A.]]
+[[Category: Bardwell, J C.A.]]
 [[Category: Bugl, H.]]
-[[Category: Fauman, E.B.]]
+[[Category: Fauman, E B.]]
 [[Category: Jakob, U.]]
-[[Category: Kushner, S.R.]]
+[[Category: Kushner, S R.]]
-[[Category: Saper, M.A.]]
+[[Category: Saper, M A.]]
-[[Category: Staker, B.L.]]
+[[Category: Staker, B L.]]
 [[Category: Zheng, F.]]
 [[Category: HG]]
@@ Line 30: / Line 30: @@
 [[Category: methyltransferase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:02:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:15 2008''