1eqn: Difference between revisions

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[[Image:1eqn.gif|left|200px]]<br /><applet load="1eqn" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1eqn.gif|left|200px]]<br /><applet load="1eqn" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1eqn, resolution 2.9Å" />

'''E.COLI PRIMASE CATALYTIC CORE'''<br />

==Overview==

Primases synthesize short RNA strands on single-stranded DNA templates, thereby generating the hybrid duplexes required for the initiation of, synthesis by DNA polymerases. We present the crystal structure of the, catalytic unit of a primase enzyme, that of a approximately 320 residue, fragment of Escherichia coli primase, determined at 2.9 A resolution., Central to the catalytic unit is a TOPRIM domain that is strikingly, similar in its structure to that of corresponding domains in DNA, topoisomerases, but is unrelated to the catalytic centers of other DNA or, RNA polymerases. The catalytic domain of primase is crescent-shaped, and, the concave face of the crescent is predicted to accommodate about 10, base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting, processivity.

Primases synthesize short RNA strands on single-stranded DNA templates, thereby generating the hybrid duplexes required for the initiation of synthesis by DNA polymerases. We present the crystal structure of the catalytic unit of a primase enzyme, that of a approximately 320 residue fragment of Escherichia coli primase, determined at 2.9 A resolution. Central to the catalytic unit is a TOPRIM domain that is strikingly similar in its structure to that of corresponding domains in DNA topoisomerases, but is unrelated to the catalytic centers of other DNA or RNA polymerases. The catalytic domain of primase is crescent-shaped, and the concave face of the crescent is predicted to accommodate about 10 base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting processivity.

==About this Structure==

1EQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1EQN OCA].

1EQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQN OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Donnell, M.O.]]

[[Category: Donnell, M O.]]

[[Category: Kuriyan, J.]]

[[Category: McInerney, P.]]

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[[Category: toprim domain]]

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@@ Line 1: / Line 1: @@
-[[Image:1eqn.gif|left|200px]]<br /><applet load="1eqn" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1eqn.gif|left|200px]]<br /><applet load="1eqn" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1eqn, resolution 2.9&Aring;" />
 '''E.COLI PRIMASE CATALYTIC CORE'''<br />
 ==Overview==
-Primases synthesize short RNA strands on single-stranded DNA templates, thereby generating the hybrid duplexes required for the initiation of, synthesis by DNA polymerases. We present the crystal structure of the, catalytic unit of a primase enzyme, that of a approximately 320 residue, fragment of Escherichia coli primase, determined at 2.9 A resolution., Central to the catalytic unit is a TOPRIM domain that is strikingly, similar in its structure to that of corresponding domains in DNA, topoisomerases, but is unrelated to the catalytic centers of other DNA or, RNA polymerases. The catalytic domain of primase is crescent-shaped, and, the concave face of the crescent is predicted to accommodate about 10, base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting, processivity.
+Primases synthesize short RNA strands on single-stranded DNA templates, thereby generating the hybrid duplexes required for the initiation of synthesis by DNA polymerases. We present the crystal structure of the catalytic unit of a primase enzyme, that of a approximately 320 residue fragment of Escherichia coli primase, determined at 2.9 A resolution. Central to the catalytic unit is a TOPRIM domain that is strikingly similar in its structure to that of corresponding domains in DNA topoisomerases, but is unrelated to the catalytic centers of other DNA or RNA polymerases. The catalytic domain of primase is crescent-shaped, and the concave face of the crescent is predicted to accommodate about 10 base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting processivity.
 ==About this Structure==
-EQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EQN OCA].
+EQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQN OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Donnell, M.O.]]
+[[Category: Donnell, M O.]]
 [[Category: Kuriyan, J.]]
 [[Category: McInerney, P.]]
@@ Line 20: / Line 20: @@
 [[Category: toprim domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:14:02 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:32 2008''