1fgz: Difference between revisions

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[[Image:1fgz.jpg|left|200px]]<br /><applet load="1fgz" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1fgz.jpg|left|200px]]<br /><applet load="1fgz" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1fgz, resolution 2.05Å" />

'''GRP1 PH DOMAIN (UNLIGANDED)'''<br />

==Overview==

Lipid second messengers generated by phosphoinositide (PI) 3-kinases, regulate diverse cellular functions through interaction with pleckstrin, homology (PH) domains in modular signaling proteins. The PH domain of, Grp1, a PI 3-kinase-activated exchange factor for Arf GTPases, selectively, binds phosphatidylinositol 3,4,5-trisphosphate with high affinity. We have, determined the structure of the Grp1 PH domain in the unliganded form and, bound to inositol 1,3,4,5-tetraphosphate. A novel mode of phosphoinositide, recognition involving a 20-residue insertion within the beta6/beta7 loop, explains the unusually high specificity of the Grp1 PH domain and the, promiscuous 3-phosphoinositide binding typical of several PH domains, including that of protein kinase B. When compared to other PH domains, general determinants of 3-phosphoinositide recognition and specificity can, be deduced.

Lipid second messengers generated by phosphoinositide (PI) 3-kinases regulate diverse cellular functions through interaction with pleckstrin homology (PH) domains in modular signaling proteins. The PH domain of Grp1, a PI 3-kinase-activated exchange factor for Arf GTPases, selectively binds phosphatidylinositol 3,4,5-trisphosphate with high affinity. We have determined the structure of the Grp1 PH domain in the unliganded form and bound to inositol 1,3,4,5-tetraphosphate. A novel mode of phosphoinositide recognition involving a 20-residue insertion within the beta6/beta7 loop explains the unusually high specificity of the Grp1 PH domain and the promiscuous 3-phosphoinositide binding typical of several PH domains including that of protein kinase B. When compared to other PH domains, general determinants of 3-phosphoinositide recognition and specificity can be deduced.

==About this Structure==

1FGZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1FGZ OCA].

1FGZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGZ OCA].

==Reference==

Line 16:

[[Category: Chawla, A.]]

[[Category: Cronin, T.]]

[[Category: Czech, M.P.]]

[[Category: Czech, M P.]]

[[Category: Klarlund, J.]]

[[Category: Lambright, D.G.]]

[[Category: Lambright, D G.]]

[[Category: Lietzke, S.E.]]

[[Category: Lietzke, S E.]]

[[Category: SO4]]

[[Category: ph domain]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:33 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1fgz.jpg|left|200px]]<br /><applet load="1fgz" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fgz.jpg|left|200px]]<br /><applet load="1fgz" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1fgz, resolution 2.05&Aring;" />
 '''GRP1 PH DOMAIN (UNLIGANDED)'''<br />
 ==Overview==
-Lipid second messengers generated by phosphoinositide (PI) 3-kinases, regulate diverse cellular functions through interaction with pleckstrin, homology (PH) domains in modular signaling proteins. The PH domain of, Grp1, a PI 3-kinase-activated exchange factor for Arf GTPases, selectively, binds phosphatidylinositol 3,4,5-trisphosphate with high affinity. We have, determined the structure of the Grp1 PH domain in the unliganded form and, bound to inositol 1,3,4,5-tetraphosphate. A novel mode of phosphoinositide, recognition involving a 20-residue insertion within the beta6/beta7 loop, explains the unusually high specificity of the Grp1 PH domain and the, promiscuous 3-phosphoinositide binding typical of several PH domains, including that of protein kinase B. When compared to other PH domains, general determinants of 3-phosphoinositide recognition and specificity can, be deduced.
+Lipid second messengers generated by phosphoinositide (PI) 3-kinases regulate diverse cellular functions through interaction with pleckstrin homology (PH) domains in modular signaling proteins. The PH domain of Grp1, a PI 3-kinase-activated exchange factor for Arf GTPases, selectively binds phosphatidylinositol 3,4,5-trisphosphate with high affinity. We have determined the structure of the Grp1 PH domain in the unliganded form and bound to inositol 1,3,4,5-tetraphosphate. A novel mode of phosphoinositide recognition involving a 20-residue insertion within the beta6/beta7 loop explains the unusually high specificity of the Grp1 PH domain and the promiscuous 3-phosphoinositide binding typical of several PH domains including that of protein kinase B. When compared to other PH domains, general determinants of 3-phosphoinositide recognition and specificity can be deduced.
 ==About this Structure==
-FGZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FGZ OCA].
+FGZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGZ OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Chawla, A.]]
 [[Category: Cronin, T.]]
-[[Category: Czech, M.P.]]
+[[Category: Czech, M P.]]
 [[Category: Klarlund, J.]]
-[[Category: Lambright, D.G.]]
+[[Category: Lambright, D G.]]
-[[Category: Lietzke, S.E.]]
+[[Category: Lietzke, S E.]]
 [[Category: SO4]]
 [[Category: ph domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:55:10 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:33 2008''