1flm: Difference between revisions

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[[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1flm, resolution 1.30Å" />

'''DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)'''<br />

==Overview==

The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio, vulgaris Miyazaki F was solved by the multiple isomorphous replacement, method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp, exists in a dimeric form in the crystal, in contrast to the monomeric, structure determined by NMR. R.m.s. deviations between the crystal, structure and the solution structure are more than 2 A, which implies, significant differences. There are some hydrophobic residues in the, interface between the two monomers. In particular, Leu122 in the, C-terminus has a close contact with the o-xylene moiety of FMN, while, solvent molecules may cover the o-xylene moiety in the solution structure.

The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio vulgaris Miyazaki F was solved by the multiple isomorphous replacement method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp exists in a dimeric form in the crystal, in contrast to the monomeric structure determined by NMR. R.m.s. deviations between the crystal structure and the solution structure are more than 2 A, which implies significant differences. There are some hydrophobic residues in the interface between the two monomers. In particular, Leu122 in the C-terminus has a close contact with the o-xylene moiety of FMN, while solvent molecules may cover the o-xylene moiety in the solution structure.

==About this Structure==

1FLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1FLM OCA].

1FLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLM OCA].

==Reference==

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[[Category: fmn binding]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 15~~:01:~~17 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:55 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1flm, resolution 1.30&Aring;" />
 '''DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)'''<br />
 ==Overview==
-The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio, vulgaris Miyazaki F was solved by the multiple isomorphous replacement, method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp, exists in a dimeric form in the crystal, in contrast to the monomeric, structure determined by NMR. R.m.s. deviations between the crystal, structure and the solution structure are more than 2 A, which implies, significant differences. There are some hydrophobic residues in the, interface between the two monomers. In particular, Leu122 in the, C-terminus has a close contact with the o-xylene moiety of FMN, while, solvent molecules may cover the o-xylene moiety in the solution structure.
+The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio vulgaris Miyazaki F was solved by the multiple isomorphous replacement method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp exists in a dimeric form in the crystal, in contrast to the monomeric structure determined by NMR. R.m.s. deviations between the crystal structure and the solution structure are more than 2 A, which implies significant differences. There are some hydrophobic residues in the interface between the two monomers. In particular, Leu122 in the C-terminus has a close contact with the o-xylene moiety of FMN, while solvent molecules may cover the o-xylene moiety in the solution structure.
 ==About this Structure==
-FLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FLM OCA].
+FLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLM OCA].
 ==Reference==
@@ Line 24: / Line 24: @@
 [[Category: fmn binding]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:01:17 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:55 2008''