1g0i: Difference between revisions
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New page: left|200px<br /><applet load="1g0i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g0i, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ... |
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[[Image:1g0i.jpg|left|200px]]<br /><applet load="1g0i" size=" | [[Image:1g0i.jpg|left|200px]]<br /><applet load="1g0i" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1g0i, resolution 2.4Å" /> | caption="1g0i, resolution 2.4Å" /> | ||
'''CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE-FRUCTOSE 1,6 BISPHOSPHATASE'''<br /> | '''CRYSTAL STRUCTURE OF MJ0109 GENE PRODUCT INOSITOL MONOPHOSPHATASE-FRUCTOSE 1,6 BISPHOSPHATASE'''<br /> | ||
==Overview== | ==Overview== | ||
Inositol monophosphatase (EC 3.1.3.25) in hyperthermophilic archaea is | Inositol monophosphatase (EC 3.1.3.25) in hyperthermophilic archaea is thought to play a role in the biosynthesis of di-myo-inositol-1,1'-phosphate (DIP), an osmolyte unique to hyperthermophiles. The Methanococcus jannaschii MJ109 gene product, the sequence of which is substantially homologous to that of human inositol monophosphatase, exhibits inositol monophosphatase activity but with substrate specificity that is broader than those of bacterial and eukaryotic inositol monophosphatases (it can also act as a fructose bisphosphatase). To understand its substrate specificity as well as the poor inhibition by Li(+) (a potent inhibitor of the mammalian enzyme), we have crystallized the enzyme and determined its three-dimensional structure. The overall fold, as expected, is similar to that of the mammalian enzyme, but the details suggest a closer relationship to fructose 1,6-bisphosphatases. Three complexes of the MJ0109 protein with substrate and/or product and inhibitory as well as activating metal ions suggest that the phosphatase mechanism is a three-metal ion assisted catalysis which is in variance with that proposed previously for the human inositol monophosphatase. | ||
==About this Structure== | ==About this Structure== | ||
1G0I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MN, PO4 and INS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-phosphate_phosphatase Inositol-phosphate phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.25 3.1.3.25] Full crystallographic information is available from [http:// | 1G0I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=INS:'>INS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-phosphate_phosphatase Inositol-phosphate phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.25 3.1.3.25] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G0I OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, L.]] | [[Category: Chen, L.]] | ||
[[Category: Johnson, K | [[Category: Johnson, K A.]] | ||
[[Category: Roberts, M | [[Category: Roberts, M F.]] | ||
[[Category: Stec, B.]] | [[Category: Stec, B.]] | ||
[[Category: Yang, H.]] | [[Category: Yang, H.]] | ||
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[[Category: inositol]] | [[Category: inositol]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:38 2008'' | ||
