1gcb: Difference between revisions
New page: left|200px<br /><applet load="1gcb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gcb, resolution 2.2Å" /> '''GAL6, YEAST BLEOMYCIN... |
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[[Image:1gcb.jpg|left|200px]]<br /><applet load="1gcb" size=" | [[Image:1gcb.jpg|left|200px]]<br /><applet load="1gcb" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1gcb, resolution 2.2Å" /> | caption="1gcb, resolution 2.2Å" /> | ||
'''GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)'''<br /> | '''GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)'''<br /> | ||
==Overview== | ==Overview== | ||
Bleomycin hydrolase is a cysteine protease that hydrolyzes the anticancer | Bleomycin hydrolase is a cysteine protease that hydrolyzes the anticancer drug bleomycin. The homolog in yeast, Gal6, has recently been identified and found to bind DNA and to act as a repressor in the Gal4 regulatory system. The crystal structure of Gal6 at 2.2 A resolution reveals a hexameric structure with a prominent central channel. The papain-like active sites are situated within the central channel, in a manner resembling the organization of active sites in the proteasome. The Gal6 channel is lined with 60 lysine residues from the six subunits, suggesting a role in DNA binding. The carboxyl-terminal arm of Gal6 extends into the active site cleft and may serve a regulatory function. Rather than each residing in distinct, separable domains, the protease and DNA-binding activities appear structurally intertwined in the hexamer, implying a coupling of these two activities. | ||
==About this Structure== | ==About this Structure== | ||
1GCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4, HG and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1GCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Johnston, S | [[Category: Johnston, S A.]] | ||
[[Category: Joshua-Tor, L.]] | [[Category: Joshua-Tor, L.]] | ||
[[Category: Rees, D | [[Category: Rees, D C.]] | ||
[[Category: Xu, H | [[Category: Xu, H E.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: HG]] | [[Category: HG]] | ||
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[[Category: ring protein]] | [[Category: ring protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:48:36 2008'' | ||
Revision as of 10:48, 21 February 2008
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GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)
Overview
Bleomycin hydrolase is a cysteine protease that hydrolyzes the anticancer drug bleomycin. The homolog in yeast, Gal6, has recently been identified and found to bind DNA and to act as a repressor in the Gal4 regulatory system. The crystal structure of Gal6 at 2.2 A resolution reveals a hexameric structure with a prominent central channel. The papain-like active sites are situated within the central channel, in a manner resembling the organization of active sites in the proteasome. The Gal6 channel is lined with 60 lysine residues from the six subunits, suggesting a role in DNA binding. The carboxyl-terminal arm of Gal6 extends into the active site cleft and may serve a regulatory function. Rather than each residing in distinct, separable domains, the protease and DNA-binding activities appear structurally intertwined in the hexamer, implying a coupling of these two activities.
About this Structure
1GCB is a Single protein structure of sequence from Saccharomyces cerevisiae with SO4, HG and GOL as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a conserved protease that binds DNA: the bleomycin hydrolase, Gal6., Joshua-Tor L, Xu HE, Johnston SA, Rees DC, Science. 1995 Aug 18;269(5226):945-50. PMID:7638617
Page seeded by OCA on Thu Feb 21 12:48:36 2008
