1gyj: Difference between revisions

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[[Image:1gyj.jpg|left|200px]]<br /><applet load="1gyj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1gyj.jpg|left|200px]]<br /><applet load="1gyj" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1gyj, resolution 2.1Å" />

'''THE CRYSTAL STRUCTURE OF YDCE, A 4-OXALOCROTONATE TAUTOMERASE HOMOLOGUE FROM ESCHERICHIA COLI, CONFIRMS THE STRUCTURAL BASIS FOR OLIGOMER DIVERSITY'''<br />

==Overview==

The tautomerase superfamily consists of three major families represented, by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), and macrophage, migration inhibitory factor (MIF). The members of this superfamily are, structurally homologous proteins constructed from a simple beta-alpha-beta, fold that share a key mechanistic feature; they use an amino-terminal, proline, which has an unusually low pK(a), as the general base in a, keto-enol tautomerization. Several new members of the 4-OT family have now, been identified using PSI-BLAST and categorized into five subfamilies on, the basis of multiple-sequence alignments and the conservation of key, catalytic and structural residues. The members of subfamily 5, which, includes a hypothetical protein designated YdcE from Escherichia coli, are, predicted not to form hexamers. The crystal structure of YdcE has been, determined to 1.35 A resolution and confirms that it is a dimer. In, addition, YdcE complexed with (E)-2-fluoro-p-hydroxycinnamate, identified, as a potent competitive inhibitor of this enzyme, as well as, N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES) and benzoate, are also presented. These latter crystal structures reveal the location of, the active site and suggest a mechanism for the observed YdcE-catalyzed, tautomerization reaction. The dimeric arrangement of YdcE represents a new, structure in the 4-OT family and demonstrates structural diversity within, the 4-OT family not previously reported.

The tautomerase superfamily consists of three major families represented by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), and macrophage migration inhibitory factor (MIF). The members of this superfamily are structurally homologous proteins constructed from a simple beta-alpha-beta fold that share a key mechanistic feature; they use an amino-terminal proline, which has an unusually low pK(a), as the general base in a keto-enol tautomerization. Several new members of the 4-OT family have now been identified using PSI-BLAST and categorized into five subfamilies on the basis of multiple-sequence alignments and the conservation of key catalytic and structural residues. The members of subfamily 5, which includes a hypothetical protein designated YdcE from Escherichia coli, are predicted not to form hexamers. The crystal structure of YdcE has been determined to 1.35 A resolution and confirms that it is a dimer. In addition, YdcE complexed with (E)-2-fluoro-p-hydroxycinnamate, identified as a potent competitive inhibitor of this enzyme, as well as N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES) and benzoate are also presented. These latter crystal structures reveal the location of the active site and suggest a mechanism for the observed YdcE-catalyzed tautomerization reaction. The dimeric arrangement of YdcE represents a new structure in the 4-OT family and demonstrates structural diversity within the 4-OT family not previously reported.

==About this Structure==

1GYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Phenylpyruvate_tautomerase Phenylpyruvate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.1 5.3.2.1] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1GYJ OCA].

1GYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Phenylpyruvate_tautomerase Phenylpyruvate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.1 5.3.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GYJ OCA].

==Reference==

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[[Category: tautomerase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 16~~:20:~~53 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:22 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1gyj.jpg|left|200px]]<br /><applet load="1gyj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1gyj.jpg|left|200px]]<br /><applet load="1gyj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1gyj, resolution 2.1&Aring;" />
 '''THE CRYSTAL STRUCTURE OF YDCE, A 4-OXALOCROTONATE TAUTOMERASE HOMOLOGUE FROM ESCHERICHIA COLI, CONFIRMS THE STRUCTURAL BASIS FOR OLIGOMER DIVERSITY'''<br />
 ==Overview==
-The tautomerase superfamily consists of three major families represented, by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), and macrophage, migration inhibitory factor (MIF). The members of this superfamily are, structurally homologous proteins constructed from a simple beta-alpha-beta, fold that share a key mechanistic feature; they use an amino-terminal, proline, which has an unusually low pK(a), as the general base in a, keto-enol tautomerization. Several new members of the 4-OT family have now, been identified using PSI-BLAST and categorized into five subfamilies on, the basis of multiple-sequence alignments and the conservation of key, catalytic and structural residues. The members of subfamily 5, which, includes a hypothetical protein designated YdcE from Escherichia coli, are, predicted not to form hexamers. The crystal structure of YdcE has been, determined to 1.35 A resolution and confirms that it is a dimer. In, addition, YdcE complexed with (E)-2-fluoro-p-hydroxycinnamate, identified, as a potent competitive inhibitor of this enzyme, as well as, N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES) and benzoate, are also presented. These latter crystal structures reveal the location of, the active site and suggest a mechanism for the observed YdcE-catalyzed, tautomerization reaction. The dimeric arrangement of YdcE represents a new, structure in the 4-OT family and demonstrates structural diversity within, the 4-OT family not previously reported.
+The tautomerase superfamily consists of three major families represented by 4-oxalocrotonate tautomerase (4-OT), 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), and macrophage migration inhibitory factor (MIF). The members of this superfamily are structurally homologous proteins constructed from a simple beta-alpha-beta fold that share a key mechanistic feature; they use an amino-terminal proline, which has an unusually low pK(a), as the general base in a keto-enol tautomerization. Several new members of the 4-OT family have now been identified using PSI-BLAST and categorized into five subfamilies on the basis of multiple-sequence alignments and the conservation of key catalytic and structural residues. The members of subfamily 5, which includes a hypothetical protein designated YdcE from Escherichia coli, are predicted not to form hexamers. The crystal structure of YdcE has been determined to 1.35 A resolution and confirms that it is a dimer. In addition, YdcE complexed with (E)-2-fluoro-p-hydroxycinnamate, identified as a potent competitive inhibitor of this enzyme, as well as N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid (HEPES) and benzoate are also presented. These latter crystal structures reveal the location of the active site and suggest a mechanism for the observed YdcE-catalyzed tautomerization reaction. The dimeric arrangement of YdcE represents a new structure in the 4-OT family and demonstrates structural diversity within the 4-OT family not previously reported.
 ==About this Structure==
-GYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Phenylpyruvate_tautomerase Phenylpyruvate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.1 5.3.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GYJ OCA].
+GYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Phenylpyruvate_tautomerase Phenylpyruvate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.1 5.3.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GYJ OCA].
 ==Reference==
@@ Line 27: / Line 27: @@
 [[Category: tautomerase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:20:53 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:22 2008''