1h2d: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1h2d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h2d, resolution 2.60Å" /> '''EBOLA VIRUS MATRIX P... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1h2d.gif|left|200px]]<br /><applet load="1h2d" size=" | [[Image:1h2d.gif|left|200px]]<br /><applet load="1h2d" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1h2d, resolution 2.60Å" /> | caption="1h2d, resolution 2.60Å" /> | ||
'''EBOLA VIRUS MATRIX PROTEIN VP40 N-TERMINAL DOMAIN IN COMPLEX WITH RNA (LOW-RESOLUTION VP40[31-212] VARIANT).'''<br /> | '''EBOLA VIRUS MATRIX PROTEIN VP40 N-TERMINAL DOMAIN IN COMPLEX WITH RNA (LOW-RESOLUTION VP40[31-212] VARIANT).'''<br /> | ||
==Overview== | ==Overview== | ||
The Ebola virus membrane-associated matrix protein VP40 is thought to be | The Ebola virus membrane-associated matrix protein VP40 is thought to be crucial for assembly and budding of virus particles. Here we present the crystal structure of a disk-shaped octameric form of VP40 formed by four antiparallel homodimers of the N-terminal domain. The octamer binds an RNA triribonucleotide containing the sequence 5'-U-G-A-3' through its inner pore surface, and its oligomerization and RNA binding properties are facilitated by two conformational changes when compared to monomeric VP40. The selective RNA interaction stabilizes the ring structure and confers in vitro SDS resistance to octameric VP40. SDS-resistant octameric VP40 is also found in Ebola virus-infected cells, which suggests that VP40 has an additional function in the life cycle of the virus besides promoting virus assembly and budding off the plasma membrane. | ||
==About this Structure== | ==About this Structure== | ||
1H2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Ebola_virus_sp. Ebola virus sp.] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1H2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Ebola_virus_sp. Ebola virus sp.] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2D OCA]. | ||
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Bracher, A.]] | [[Category: Bracher, A.]] | ||
[[Category: Dessen, A.]] | [[Category: Dessen, A.]] | ||
[[Category: Gomis-Ruth, F | [[Category: Gomis-Ruth, F X.]] | ||
[[Category: Klenk, H | [[Category: Klenk, H D.]] | ||
[[Category: Weissenhorn, W.]] | [[Category: Weissenhorn, W.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
| Line 26: | Line 26: | ||
[[Category: matrix protein vp40]] | [[Category: matrix protein vp40]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:35 2008'' | ||
