1h34: Difference between revisions

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[[Image:1h34.jpg|left|200px]]<br /><applet load="1h34" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1h34.jpg|left|200px]]<br /><applet load="1h34" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1h34, resolution 2.04Å" />

'''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR'''<br />

==Overview==

SAD (single-wavelength anomalous diffraction) has enormous potential for, phasing proteins using only the anomalous signal of the almost ubiquitous, native sulfur, but requires extremely precise data. The previously unknown, structure of the lima bean trypsin inhibitor (LBTI) was solved using, highly redundant data collected to 3 A using a CCD detector with a, rotating-anode generator and three-circle goniometer. The seven 'super-S', atoms (disulfide bridges) were located by dual-space recycling with SHELXD, and the high solvent content enabled the density-modification program, SHELXE to generate high-quality maps despite the modest resolution., Subsequently, a 2.05 A synchrotron data set was collected and used for, further phase extension and structure refinement.

SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.

==About this Structure==

1H34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA].

1H34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Bunkoczi, G.]]

[[Category: Debreczeni, J.E.]]

[[Category: Debreczeni, J E.]]

[[Category: Girmann, B.]]

[[Category: Sheldrick, G.M.]]

[[Category: Sheldrick, G M.]]

[[Category: bowman-birk-type proteinase inhibitor]]

[[Category: serine protease inhibitor]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 16~~:24:~~19 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:51 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1h34.jpg|left|200px]]<br /><applet load="1h34" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1h34.jpg|left|200px]]<br /><applet load="1h34" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1h34, resolution 2.04&Aring;" />
 '''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR'''<br />
 ==Overview==
-SAD (single-wavelength anomalous diffraction) has enormous potential for, phasing proteins using only the anomalous signal of the almost ubiquitous, native sulfur, but requires extremely precise data. The previously unknown, structure of the lima bean trypsin inhibitor (LBTI) was solved using, highly redundant data collected to 3 A using a CCD detector with a, rotating-anode generator and three-circle goniometer. The seven 'super-S', atoms (disulfide bridges) were located by dual-space recycling with SHELXD, and the high solvent content enabled the density-modification program, SHELXE to generate high-quality maps despite the modest resolution., Subsequently, a 2.05 A synchrotron data set was collected and used for, further phase extension and structure refinement.
+SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.
 ==About this Structure==
-H34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA].
+H34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Bunkoczi, G.]]
-[[Category: Debreczeni, J.E.]]
+[[Category: Debreczeni, J E.]]
 [[Category: Girmann, B.]]
-[[Category: Sheldrick, G.M.]]
+[[Category: Sheldrick, G M.]]
 [[Category: bowman-birk-type proteinase inhibitor]]
 [[Category: serine protease inhibitor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:24:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:51 2008''