1i17: Difference between revisions

Line 1:

[[Image:1i17.gif|left|200px]]<br /><applet load="1i17" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1i17.gif|left|200px]]<br /><applet load="1i17" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1i17" />

'''NMR STRUCTURE OF MOUSE DOPPEL 51-157'''<br />

==Overview==

The downstream prion-like protein (doppel, or Dpl) is a paralog of the, cellular prion protein, PrP(C). The two proteins have approximately 25%, sequence identity, but seem to have distinct physiologic roles. Unlike, PrP(C), Dpl does not support prion replication; instead, overexpression of, Dpl in the brain seems to cause a completely different neurodegenerative, disease. We report the solution structure of a fragment of recombinant, mouse Dpl (residues 26-157) containing a globular domain with three, helices and a small amount of beta-structure. Overall, the topology of Dpl, is very similar to that of PrP(C). Significant differences include a, marked kink in one of the helices in Dpl, and a different orientation of, the two short beta-strands. Although the two proteins most likely arose, through duplication of a single ancestral gene, the relationship is now so, distant that only the structures retain similarity; the functions have, diversified along with the sequence.

The downstream prion-like protein (doppel, or Dpl) is a paralog of the cellular prion protein, PrP(C). The two proteins have approximately 25% sequence identity, but seem to have distinct physiologic roles. Unlike PrP(C), Dpl does not support prion replication; instead, overexpression of Dpl in the brain seems to cause a completely different neurodegenerative disease. We report the solution structure of a fragment of recombinant mouse Dpl (residues 26-157) containing a globular domain with three helices and a small amount of beta-structure. Overall, the topology of Dpl is very similar to that of PrP(C). Significant differences include a marked kink in one of the helices in Dpl, and a different orientation of the two short beta-strands. Although the two proteins most likely arose through duplication of a single ancestral gene, the relationship is now so distant that only the structures retain similarity; the functions have diversified along with the sequence.

==About this Structure==

1I17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1I17 OCA].

1I17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I17 OCA].

==Reference==

Line 13:

[[Category: Mus musculus]]

[[Category: Single protein]]

[[Category: Cohen, F.E.]]

[[Category: Cohen, F E.]]

[[Category: Dyson, H.J.]]

[[Category: Dyson, H J.]]

[[Category: Mo, H.]]

[[Category: Moore, R.C.]]

[[Category: Moore, R C.]]

[[Category: Prusiner, S.B.]]

[[Category: Prusiner, S B.]]

[[Category: Westaway, D.]]

[[Category: Wright, P.E.]]

[[Category: Wright, P E.]]

[[Category: doppel]]

[[Category: dpl]]

Line 25:

[[Category: prion]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 16~~:57:~~59 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:06:53 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1i17.gif|left|200px]]<br /><applet load="1i17" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1i17.gif|left|200px]]<br /><applet load="1i17" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1i17" />
 '''NMR STRUCTURE OF MOUSE DOPPEL 51-157'''<br />
 ==Overview==
-The downstream prion-like protein (doppel, or Dpl) is a paralog of the, cellular prion protein, PrP(C). The two proteins have approximately 25%, sequence identity, but seem to have distinct physiologic roles. Unlike, PrP(C), Dpl does not support prion replication; instead, overexpression of, Dpl in the brain seems to cause a completely different neurodegenerative, disease. We report the solution structure of a fragment of recombinant, mouse Dpl (residues 26-157) containing a globular domain with three, helices and a small amount of beta-structure. Overall, the topology of Dpl, is very similar to that of PrP(C). Significant differences include a, marked kink in one of the helices in Dpl, and a different orientation of, the two short beta-strands. Although the two proteins most likely arose, through duplication of a single ancestral gene, the relationship is now so, distant that only the structures retain similarity; the functions have, diversified along with the sequence.
+The downstream prion-like protein (doppel, or Dpl) is a paralog of the cellular prion protein, PrP(C). The two proteins have approximately 25% sequence identity, but seem to have distinct physiologic roles. Unlike PrP(C), Dpl does not support prion replication; instead, overexpression of Dpl in the brain seems to cause a completely different neurodegenerative disease. We report the solution structure of a fragment of recombinant mouse Dpl (residues 26-157) containing a globular domain with three helices and a small amount of beta-structure. Overall, the topology of Dpl is very similar to that of PrP(C). Significant differences include a marked kink in one of the helices in Dpl, and a different orientation of the two short beta-strands. Although the two proteins most likely arose through duplication of a single ancestral gene, the relationship is now so distant that only the structures retain similarity; the functions have diversified along with the sequence.
 ==About this Structure==
-I17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I17 OCA].
+I17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I17 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Mus musculus]]
 [[Category: Single protein]]
-[[Category: Cohen, F.E.]]
+[[Category: Cohen, F E.]]
-[[Category: Dyson, H.J.]]
+[[Category: Dyson, H J.]]
 [[Category: Mo, H.]]
-[[Category: Moore, R.C.]]
+[[Category: Moore, R C.]]
-[[Category: Prusiner, S.B.]]
+[[Category: Prusiner, S B.]]
 [[Category: Westaway, D.]]
-[[Category: Wright, P.E.]]
+[[Category: Wright, P E.]]
 [[Category: doppel]]
 [[Category: dpl]]
@@ Line 25: / Line 25: @@
 [[Category: prion]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:57:59 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:06:53 2008''