1i7e: Difference between revisions

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[[Image:1i7e.gif|left|200px]]<br /><applet load="1i7e" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1i7e.gif|left|200px]]<br /><applet load="1i7e" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1i7e, resolution 1.95Å" />

'''C-Terminal Domain Of Mouse Brain Tubby Protein bound to Phosphatidylinositol 4,5-bis-phosphate'''<br />

==Overview==

Dysfunction of the tubby protein results in maturity-onset obesity in, mice. Tubby has been implicated as a transcription regulator, but details, of the molecular mechanism underlying its function remain unclear. Here we, show that tubby functions in signal transduction from heterotrimeric, GTP-binding protein (G protein)-coupled receptors. Tubby localizes to the, plasma membrane by binding phosphatidylinositol 4,5-bisphosphate through, its carboxyl terminal "tubby domain." X-ray crystallography reveals the, atomic-level basis of this interaction and implicates tubby domains as, phosphorylated-phosphatidyl- inositol binding factors. Receptor-mediated, activation of G protein alphaq (Galphaq) releases tubby from the plasma, membrane through the action of phospholipase C-beta, triggering, translocation of tubby to the cell nucleus. The localization of tubby-like, protein 3 (TULP3) is similarly regulated. These data suggest that tubby, proteins function as membrane-bound transcription regulators that, translocate to the nucleus in response to phosphoinositide hydrolysis, providing a direct link between G-protein signaling and the regulation of, gene expression.

Dysfunction of the tubby protein results in maturity-onset obesity in mice. Tubby has been implicated as a transcription regulator, but details of the molecular mechanism underlying its function remain unclear. Here we show that tubby functions in signal transduction from heterotrimeric GTP-binding protein (G protein)-coupled receptors. Tubby localizes to the plasma membrane by binding phosphatidylinositol 4,5-bisphosphate through its carboxyl terminal "tubby domain." X-ray crystallography reveals the atomic-level basis of this interaction and implicates tubby domains as phosphorylated-phosphatidyl- inositol binding factors. Receptor-mediated activation of G protein alphaq (Galphaq) releases tubby from the plasma membrane through the action of phospholipase C-beta, triggering translocation of tubby to the cell nucleus. The localization of tubby-like protein 3 (TULP3) is similarly regulated. These data suggest that tubby proteins function as membrane-bound transcription regulators that translocate to the nucleus in response to phosphoinositide hydrolysis, providing a direct link between G-protein signaling and the regulation of gene expression.

==About this Structure==

1I7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with IBS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1I7E OCA].

1I7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=IBS:'>IBS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7E OCA].

==Reference==

Line 13:

[[Category: Mus musculus]]

[[Category: Single protein]]

[[Category: Baird, C.L.]]

[[Category: Baird, C L.]]

[[Category: Boggon, T.J.]]

[[Category: Boggon, T J.]]

[[Category: Santagata, S.]]

[[Category: Shan, W.S.]]

[[Category: Shan, W S.]]

[[Category: Shapiro, L.]]

[[Category: IBS]]

[[Category: tubby filled-barrel beta-barrel filled-beta-roll 12-stranded-beta-barrel helix-filled-barrel obesity blindness deafness phosphoinositide phosphatidylinositol]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:07:~~19 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:45 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1i7e.gif|left|200px]]<br /><applet load="1i7e" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1i7e.gif|left|200px]]<br /><applet load="1i7e" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1i7e, resolution 1.95&Aring;" />
 '''C-Terminal Domain Of Mouse Brain Tubby Protein bound to Phosphatidylinositol 4,5-bis-phosphate'''<br />
 ==Overview==
-Dysfunction of the tubby protein results in maturity-onset obesity in, mice. Tubby has been implicated as a transcription regulator, but details, of the molecular mechanism underlying its function remain unclear. Here we, show that tubby functions in signal transduction from heterotrimeric, GTP-binding protein (G protein)-coupled receptors. Tubby localizes to the, plasma membrane by binding phosphatidylinositol 4,5-bisphosphate through, its carboxyl terminal "tubby domain." X-ray crystallography reveals the, atomic-level basis of this interaction and implicates tubby domains as, phosphorylated-phosphatidyl- inositol binding factors. Receptor-mediated, activation of G protein alphaq (Galphaq) releases tubby from the plasma, membrane through the action of phospholipase C-beta, triggering, translocation of tubby to the cell nucleus. The localization of tubby-like, protein 3 (TULP3) is similarly regulated. These data suggest that tubby, proteins function as membrane-bound transcription regulators that, translocate to the nucleus in response to phosphoinositide hydrolysis, providing a direct link between G-protein signaling and the regulation of, gene expression.
+Dysfunction of the tubby protein results in maturity-onset obesity in mice. Tubby has been implicated as a transcription regulator, but details of the molecular mechanism underlying its function remain unclear. Here we show that tubby functions in signal transduction from heterotrimeric GTP-binding protein (G protein)-coupled receptors. Tubby localizes to the plasma membrane by binding phosphatidylinositol 4,5-bisphosphate through its carboxyl terminal "tubby domain." X-ray crystallography reveals the atomic-level basis of this interaction and implicates tubby domains as phosphorylated-phosphatidyl- inositol binding factors. Receptor-mediated activation of G protein alphaq (Galphaq) releases tubby from the plasma membrane through the action of phospholipase C-beta, triggering translocation of tubby to the cell nucleus. The localization of tubby-like protein 3 (TULP3) is similarly regulated. These data suggest that tubby proteins function as membrane-bound transcription regulators that translocate to the nucleus in response to phosphoinositide hydrolysis, providing a direct link between G-protein signaling and the regulation of gene expression.
 ==About this Structure==
-I7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with IBS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I7E OCA].
+I7E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=IBS:'>IBS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7E OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Mus musculus]]
 [[Category: Single protein]]
-[[Category: Baird, C.L.]]
+[[Category: Baird, C L.]]
-[[Category: Boggon, T.J.]]
+[[Category: Boggon, T J.]]
 [[Category: Santagata, S.]]
-[[Category: Shan, W.S.]]
+[[Category: Shan, W S.]]
 [[Category: Shapiro, L.]]
 [[Category: IBS]]
 [[Category: tubby filled-barrel beta-barrel filled-beta-roll 12-stranded-beta-barrel helix-filled-barrel obesity blindness deafness phosphoinositide phosphatidylinositol]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:07:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:45 2008''