1idp: Difference between revisions

Line 1:

[[Image:1idp.gif|left|200px]]<br /><applet load="1idp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1idp.gif|left|200px]]<br /><applet load="1idp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1idp, resolution 1.45Å" />

'''Crystal structure of scytalone dehydratase F162A mutant in the unligated state'''<br />

==Overview==

Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal, region was replaced with alanine, was crystallized with polyethylene, glycol 4000. Because the crystal was radiation-sensitive, the diffraction, data were collected at cryogenic temperatures. The crystal belonged to, monoclinic space group P2(1), with unit-cell parameters a = 72.64, b =, 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M), value was acceptable when a trimer of the mutant enzyme occupied a, crystallographic asymmetric unit. The resolution limit was extended to, 1.45 A at BL41XU of SPring-8 at 37 K.

Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K.

==About this Structure==

1IDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1IDP OCA].

1IDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDP OCA].

==Reference==

Line 20:

[[Category: melanine biosynthesis]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:19:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:52 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1idp.gif|left|200px]]<br /><applet load="1idp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1idp.gif|left|200px]]<br /><applet load="1idp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1idp, resolution 1.45&Aring;" />
 '''Crystal structure of scytalone dehydratase F162A mutant in the unligated state'''<br />
 ==Overview==
-Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal, region was replaced with alanine, was crystallized with polyethylene, glycol 4000. Because the crystal was radiation-sensitive, the diffraction, data were collected at cryogenic temperatures. The crystal belonged to, monoclinic space group P2(1), with unit-cell parameters a = 72.64, b =, 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M), value was acceptable when a trimer of the mutant enzyme occupied a, crystallographic asymmetric unit. The resolution limit was extended to, 1.45 A at BL41XU of SPring-8 at 37 K.
+Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K.
 ==About this Structure==
-IDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IDP OCA].
+IDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDP OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: melanine biosynthesis]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:19:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:52 2008''