1if0: Difference between revisions

Line 1:

[[Image:1if0.gif|left|200px]]<br /><applet load="1if0" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1if0.gif|left|200px]]<br /><applet load="1if0" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1if0, resolution 12.Å" />

'''PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)'''<br />

==Overview==

Large-scale conformational changes transform viral precursors into, infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked, topology. We have visualized its precursor, Prohead-II, by cryoelectron, microscopy and modeled the conformational change by appropriately adapting, Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching, to an entirely different set of interactions; in addition, two motifs, undergo refolding. These changes stabilize the capsid by increasing the, surface area buried at interfaces and bringing the cross-link-forming, residues, initially approximately 40 angstroms apart, close together. The, inner surface of Prohead-II is negatively charged, suggesting that the, transition is triggered electrostatically by DNA packaging.

Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially approximately 40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.

==About this Structure==

1IF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].

1IF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Cheng, N.]]

[[Category: Conway, J.F.]]

[[Category: Conway, J F.]]

[[Category: Duda, R.L.]]

[[Category: Duda, R L.]]

[[Category: Hendrix, R.W.]]

[[Category: Hendrix, R W.]]

[[Category: Johnson, J.E.]]

[[Category: Johnson, J E.]]

[[Category: Steven, A.C.]]

[[Category: Steven, A C.]]

[[Category: Wikoff, W.R.]]

[[Category: Wikoff, W R.]]

[[Category: bacteriophage]]

[[Category: capsid]]

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[[Category: virus]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:21:~~15 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:13 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1if0.gif|left|200px]]<br /><applet load="1if0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1if0.gif|left|200px]]<br /><applet load="1if0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1if0, resolution 12.&Aring;" />
 '''PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)'''<br />
 ==Overview==
-Large-scale conformational changes transform viral precursors into, infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked, topology. We have visualized its precursor, Prohead-II, by cryoelectron, microscopy and modeled the conformational change by appropriately adapting, Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching, to an entirely different set of interactions; in addition, two motifs, undergo refolding. These changes stabilize the capsid by increasing the, surface area buried at interfaces and bringing the cross-link-forming, residues, initially approximately 40 angstroms apart, close together. The, inner surface of Prohead-II is negatively charged, suggesting that the, transition is triggered electrostatically by DNA packaging.
+Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially approximately 40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.
 ==About this Structure==
-IF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].
+IF0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Cheng, N.]]
-[[Category: Conway, J.F.]]
+[[Category: Conway, J F.]]
-[[Category: Duda, R.L.]]
+[[Category: Duda, R L.]]
-[[Category: Hendrix, R.W.]]
+[[Category: Hendrix, R W.]]
-[[Category: Johnson, J.E.]]
+[[Category: Johnson, J E.]]
-[[Category: Steven, A.C.]]
+[[Category: Steven, A C.]]
-[[Category: Wikoff, W.R.]]
+[[Category: Wikoff, W R.]]
 [[Category: bacteriophage]]
 [[Category: capsid]]
@@ Line 27: / Line 27: @@
 [[Category: virus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:21:15 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:13 2008''