1ij2: Difference between revisions

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[[Image:1ij2.jpg|left|200px]]<br /><applet load="1ij2" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ij2.jpg|left|200px]]<br /><applet load="1ij2" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ij2, resolution 1.70Å" />

'''GCN4-pVTL Coiled-coil Trimer with Threonine at the a(16) position'''<br />

==Overview==

Coiled coils, estimated to constitute 3-5% of the encoded residues in most, genomes, are characterized by a heptad repeat, (abcdefg)(n), where the, buried a and d positions form the interface between multiple, alpha-helices. Although generally hydrophobic, a substantial fraction (, approximately 20%) of these a- and d-position residues are polar or, charged. We constructed variants of the well-characterized coiled coil, GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a, or a d position. The stability and oligomeric specificity of each variant, were measured, and crystal structures of coiled-coil trimers with, threonine or serine at either an a or a d position were determined. The, structures show how single polar residues in the interface affect not only, local packing, but also overall coiled-coil geometry as seen by changes in, the Crick supercoil parameters and core cavity volumes.

Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.

==About this Structure==

1IJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CD and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1IJ2 OCA].

1IJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ2 OCA].

==Reference==

Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11371197 11371197]

[[Category: Single protein]]

[[Category: Akey, D.L.]]

[[Category: Akey, D L.]]

[[Category: Kim, P.S.]]

[[Category: Kim, P S.]]

[[Category: Malashkevich, V.N.]]

[[Category: Malashkevich, V N.]]

[[Category: ACE]]

[[Category: CD]]

[[Category: gcn4 coiled coil]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:26:~~20 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:23 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ij2.jpg|left|200px]]<br /><applet load="1ij2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ij2.jpg|left|200px]]<br /><applet load="1ij2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ij2, resolution 1.70&Aring;" />
 '''GCN4-pVTL Coiled-coil Trimer with Threonine at the a(16) position'''<br />
 ==Overview==
-Coiled coils, estimated to constitute 3-5% of the encoded residues in most, genomes, are characterized by a heptad repeat, (abcdefg)(n), where the, buried a and d positions form the interface between multiple, alpha-helices. Although generally hydrophobic, a substantial fraction (, approximately 20%) of these a- and d-position residues are polar or, charged. We constructed variants of the well-characterized coiled coil, GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a, or a d position. The stability and oligomeric specificity of each variant, were measured, and crystal structures of coiled-coil trimers with, threonine or serine at either an a or a d position were determined. The, structures show how single polar residues in the interface affect not only, local packing, but also overall coiled-coil geometry as seen by changes in, the Crick supercoil parameters and core cavity volumes.
+Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.
 ==About this Structure==
-IJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CD and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IJ2 OCA].
+IJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ2 OCA].
 ==Reference==
 Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11371197 11371197]
 [[Category: Single protein]]
-[[Category: Akey, D.L.]]
+[[Category: Akey, D L.]]
-[[Category: Kim, P.S.]]
+[[Category: Kim, P S.]]
-[[Category: Malashkevich, V.N.]]
+[[Category: Malashkevich, V N.]]
 [[Category: ACE]]
 [[Category: CD]]
 [[Category: gcn4 coiled coil]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:26:20 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:23 2008''