1ij5: Difference between revisions

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[[Image:1ij5.gif|left|200px]]<br /><applet load="1ij5" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ij5.gif|left|200px]]<br /><applet load="1ij5" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ij5, resolution 3.0Å" />

'''METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD'''<br />

==Overview==

Acellular slime mold, Physarum polycephalum, has a unique wound-healing, system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large, aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N, terminus by a proteinase in plasmodia (CBP40delta), which does not, aggregate in the Ca(2+)-bound form. Here we report the crystal structures, of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both, structures consist of three domains: coiled-coil, intervening, and, EF-hand. The topology of the EF-hand domain is similar to that of calpain., The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands, through a large hydrophobic interface, necessary for high Ca(2+) affinity., Conformational change upon Ca(2+) binding is small; however, the structure, of CBP40delta provides novel insights into the mechanism of, Ca(2+)-dependent oligomerization.

Acellular slime mold, Physarum polycephalum, has a unique wound-healing system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N terminus by a proteinase in plasmodia (CBP40delta), which does not aggregate in the Ca(2+)-bound form. Here we report the crystal structures of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both structures consist of three domains: coiled-coil, intervening, and EF-hand. The topology of the EF-hand domain is similar to that of calpain. The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands through a large hydrophobic interface, necessary for high Ca(2+) affinity. Conformational change upon Ca(2+) binding is small; however, the structure of CBP40delta provides novel insights into the mechanism of Ca(2+)-dependent oligomerization.

==About this Structure==

1IJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1IJ5 OCA].

1IJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ5 OCA].

==Reference==

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[[Category: fourty kda calcium binding protein]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:26:~~28 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:23 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ij5.gif|left|200px]]<br /><applet load="1ij5" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ij5.gif|left|200px]]<br /><applet load="1ij5" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ij5, resolution 3.0&Aring;" />
 '''METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD'''<br />
 ==Overview==
-Acellular slime mold, Physarum polycephalum, has a unique wound-healing, system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large, aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N, terminus by a proteinase in plasmodia (CBP40delta), which does not, aggregate in the Ca(2+)-bound form. Here we report the crystal structures, of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both, structures consist of three domains: coiled-coil, intervening, and, EF-hand. The topology of the EF-hand domain is similar to that of calpain., The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands, through a large hydrophobic interface, necessary for high Ca(2+) affinity., Conformational change upon Ca(2+) binding is small; however, the structure, of CBP40delta provides novel insights into the mechanism of, Ca(2+)-dependent oligomerization.
+Acellular slime mold, Physarum polycephalum, has a unique wound-healing system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N terminus by a proteinase in plasmodia (CBP40delta), which does not aggregate in the Ca(2+)-bound form. Here we report the crystal structures of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both structures consist of three domains: coiled-coil, intervening, and EF-hand. The topology of the EF-hand domain is similar to that of calpain. The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands through a large hydrophobic interface, necessary for high Ca(2+) affinity. Conformational change upon Ca(2+) binding is small; however, the structure of CBP40delta provides novel insights into the mechanism of Ca(2+)-dependent oligomerization.
 ==About this Structure==
-IJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IJ5 OCA].
+IJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ5 OCA].
 ==Reference==
@@ Line 21: / Line 21: @@
 [[Category: fourty kda calcium binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:26:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:23 2008''