1ivv: Difference between revisions

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[[Image:1ivv.jpg|left|200px]]<br /><applet load="1ivv" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ivv.jpg|left|200px]]<br /><applet load="1ivv" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ivv, resolution 2.1Å" />

'''Crystal structure of copper amine oxidase from Arthrobacter globiformis: Early intermediate in topaquinone biogenesis'''<br />

==Overview==

The quinone cofactor TPQ in copper amine oxidase is generated by, posttranslational modification of an active site tyrosine residue. Using, X-ray crystallography, we have probed the copper-dependent autooxidation, process of TPQ in the enzyme from Arthrobacter globiformis. Apo enzyme, crystals were anaerobically soaked with copper; the structure determined, from this crystal provides a view of the initial state: the unmodified, tyrosine coordinated to the bound copper. Exposure of the copper-bound, crystals to oxygen led to the formation of freeze-trapped intermediates;, structural analyses indicate that these intermediates contain, dihydroxyphenylalanine quinone and trihydroxyphenylalanine. These are the, first visualized intermediates during TPQ biogenesis in copper amine, oxidase.

The quinone cofactor TPQ in copper amine oxidase is generated by posttranslational modification of an active site tyrosine residue. Using X-ray crystallography, we have probed the copper-dependent autooxidation process of TPQ in the enzyme from Arthrobacter globiformis. Apo enzyme crystals were anaerobically soaked with copper; the structure determined from this crystal provides a view of the initial state: the unmodified tyrosine coordinated to the bound copper. Exposure of the copper-bound crystals to oxygen led to the formation of freeze-trapped intermediates; structural analyses indicate that these intermediates contain dihydroxyphenylalanine quinone and trihydroxyphenylalanine. These are the first visualized intermediates during TPQ biogenesis in copper amine oxidase.

==About this Structure==

1IVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1IVV OCA].

1IVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IVV OCA].

==Reference==

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[[Category: tpq]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:42:~~42 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:16:10 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ivv.jpg|left|200px]]<br /><applet load="1ivv" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ivv.jpg|left|200px]]<br /><applet load="1ivv" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ivv, resolution 2.1&Aring;" />
 '''Crystal structure of copper amine oxidase from Arthrobacter globiformis: Early intermediate in topaquinone biogenesis'''<br />
 ==Overview==
-The quinone cofactor TPQ in copper amine oxidase is generated by, posttranslational modification of an active site tyrosine residue. Using, X-ray crystallography, we have probed the copper-dependent autooxidation, process of TPQ in the enzyme from Arthrobacter globiformis. Apo enzyme, crystals were anaerobically soaked with copper; the structure determined, from this crystal provides a view of the initial state: the unmodified, tyrosine coordinated to the bound copper. Exposure of the copper-bound, crystals to oxygen led to the formation of freeze-trapped intermediates;, structural analyses indicate that these intermediates contain, dihydroxyphenylalanine quinone and trihydroxyphenylalanine. These are the, first visualized intermediates during TPQ biogenesis in copper amine, oxidase.
+The quinone cofactor TPQ in copper amine oxidase is generated by posttranslational modification of an active site tyrosine residue. Using X-ray crystallography, we have probed the copper-dependent autooxidation process of TPQ in the enzyme from Arthrobacter globiformis. Apo enzyme crystals were anaerobically soaked with copper; the structure determined from this crystal provides a view of the initial state: the unmodified tyrosine coordinated to the bound copper. Exposure of the copper-bound crystals to oxygen led to the formation of freeze-trapped intermediates; structural analyses indicate that these intermediates contain dihydroxyphenylalanine quinone and trihydroxyphenylalanine. These are the first visualized intermediates during TPQ biogenesis in copper amine oxidase.
 ==About this Structure==
-IVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IVV OCA].
+IVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IVV OCA].
 ==Reference==
@@ Line 32: / Line 32: @@
 [[Category: tpq]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:42:42 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:16:10 2008''