1jch: Difference between revisions

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[[Image:1jch.gif|left|200px]]<br /><applet load="1jch" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1jch.gif|left|200px]]<br /><applet load="1jch" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1jch, resolution 3.02Å" />

'''Crystal Structure of Colicin E3 in Complex with its Immunity Protein'''<br />

==Overview==

Colicins kill E. coli by a process that involves binding to a surface, receptor, entering the cell, and, finally, intoxicating it. The lethal, action of colicin E3 is a specific cleavage in the ribosomal decoding A, site. The crystal structure of colicin E3, reported here in a binary, complex with its immunity protein (IP), reveals a Y-shaped molecule with, the receptor binding domain forming a 100 A long stalk and the two, globular heads of the translocation domain (T) and the catalytic domain, (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active, site, IP prevents access of the active site to the ribosome.

Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome.

==About this Structure==

1JCH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CIT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA].

1JCH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CIT:'>CIT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA].

==Reference==

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[[Category: translocation domain is a beta-jellyroll]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:05 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1jch.gif|left|200px]]<br /><applet load="1jch" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jch.gif|left|200px]]<br /><applet load="1jch" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jch, resolution 3.02&Aring;" />
 '''Crystal Structure of Colicin E3 in Complex with its Immunity Protein'''<br />
 ==Overview==
-Colicins kill E. coli by a process that involves binding to a surface, receptor, entering the cell, and, finally, intoxicating it. The lethal, action of colicin E3 is a specific cleavage in the ribosomal decoding A, site. The crystal structure of colicin E3, reported here in a binary, complex with its immunity protein (IP), reveals a Y-shaped molecule with, the receptor binding domain forming a 100 A long stalk and the two, globular heads of the translocation domain (T) and the catalytic domain, (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active, site, IP prevents access of the active site to the ribosome.
+Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome.
 ==About this Structure==
-JCH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CIT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA].
+JCH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CIT:'>CIT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA].
 ==Reference==
@@ Line 24: / Line 24: @@
 [[Category: translocation domain is a beta-jellyroll]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:06:25 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:05 2008''