1jf9: Difference between revisions
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New page: left|200px<br /><applet load="1jf9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jf9, resolution 2.Å" /> '''Crystal Structure of s... |
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[[Image:1jf9.gif|left|200px]]<br /><applet load="1jf9" size=" | [[Image:1jf9.gif|left|200px]]<br /><applet load="1jf9" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1jf9, resolution 2.Å" /> | caption="1jf9, resolution 2.Å" /> | ||
'''Crystal Structure of selenocysteine lyase'''<br /> | '''Crystal Structure of selenocysteine lyase'''<br /> | ||
==Overview== | ==Overview== | ||
The Escherichia coli NifS CsdB protein is a member of the homodimeric | The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites. | ||
==About this Structure== | ==About this Structure== | ||
1JF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PLP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] Full crystallographic information is available from [http:// | 1JF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Selenocysteine lyase]] | [[Category: Selenocysteine lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: Lima, C | [[Category: Lima, C D.]] | ||
[[Category: NYSGXRC, New | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: PLP]] | [[Category: PLP]] | ||
[[Category: cysteine]] | [[Category: cysteine]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:00 2008'' | ||
