1jj2: Difference between revisions
New page: left|200px<br /><applet load="1jj2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jj2, resolution 2.40Å" /> '''Fully Refined Crysta... |
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[[Image:1jj2.jpg|left|200px]]<br /><applet load="1jj2" size=" | [[Image:1jj2.jpg|left|200px]]<br /><applet load="1jj2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1jj2, resolution 2.40Å" /> | caption="1jj2, resolution 2.40Å" /> | ||
'''Fully Refined Crystal Structure of the Haloarcula marismortui Large Ribosomal Subunit at 2.4 Angstrom Resolution'''<br /> | '''Fully Refined Crystal Structure of the Haloarcula marismortui Large Ribosomal Subunit at 2.4 Angstrom Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
Analysis of the Haloarcula marismortui large ribosomal subunit has | Analysis of the Haloarcula marismortui large ribosomal subunit has revealed a common RNA structure that we call the kink-turn, or K-turn. The six K-turns in H.marismortui 23S rRNA superimpose with an r.m.s.d. of 1.7 A. There are two K-turns in the structure of Thermus thermophilus 16S rRNA, and the structures of U4 snRNA and L30e mRNA fragments form K-turns. The structure has a kink in the phosphodiester backbone that causes a sharp turn in the RNA helix. Its asymmetric internal loop is flanked by C-G base pairs on one side and sheared G-A base pairs on the other, with an A-minor interaction between these two helical stems. A derived consensus secondary structure for the K-turn includes 10 consensus nucleotides out of 15, and predicts its presence in the 5'-UTR of L10 mRNA, helix 78 in Escherichia coli 23S rRNA and human RNase MRP. Five K-turns in 23S rRNA interact with nine proteins. While the observed K-turns interact with proteins of unrelated structures in different ways, they interact with L7Ae and two homologous proteins in the same way. | ||
==About this Structure== | ==About this Structure== | ||
1JJ2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with MG, K, NA, CD and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1JJ2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Haloarcula marismortui]] | [[Category: Haloarcula marismortui]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Klein, D | [[Category: Klein, D J.]] | ||
[[Category: Moore, P | [[Category: Moore, P B.]] | ||
[[Category: Schmeing, T | [[Category: Schmeing, T M.]] | ||
[[Category: Steitz, T | [[Category: Steitz, T A.]] | ||
[[Category: CD]] | [[Category: CD]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: rna-rna]] | [[Category: rna-rna]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:13 2008'' | ||
Revision as of 11:23, 21 February 2008
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Fully Refined Crystal Structure of the Haloarcula marismortui Large Ribosomal Subunit at 2.4 Angstrom Resolution
Overview
Analysis of the Haloarcula marismortui large ribosomal subunit has revealed a common RNA structure that we call the kink-turn, or K-turn. The six K-turns in H.marismortui 23S rRNA superimpose with an r.m.s.d. of 1.7 A. There are two K-turns in the structure of Thermus thermophilus 16S rRNA, and the structures of U4 snRNA and L30e mRNA fragments form K-turns. The structure has a kink in the phosphodiester backbone that causes a sharp turn in the RNA helix. Its asymmetric internal loop is flanked by C-G base pairs on one side and sheared G-A base pairs on the other, with an A-minor interaction between these two helical stems. A derived consensus secondary structure for the K-turn includes 10 consensus nucleotides out of 15, and predicts its presence in the 5'-UTR of L10 mRNA, helix 78 in Escherichia coli 23S rRNA and human RNase MRP. Five K-turns in 23S rRNA interact with nine proteins. While the observed K-turns interact with proteins of unrelated structures in different ways, they interact with L7Ae and two homologous proteins in the same way.
About this Structure
1JJ2 is a Protein complex structure of sequences from Haloarcula marismortui with MG, K, NA, CD and CL as ligands. Full crystallographic information is available from OCA.
Reference
The kink-turn: a new RNA secondary structure motif., Klein DJ, Schmeing TM, Moore PB, Steitz TA, EMBO J. 2001 Aug 1;20(15):4214-21. PMID:11483524
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