1jxm: Difference between revisions

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[[Image:1jxm.jpg|left|200px]]<br /><applet load="1jxm" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1jxm.jpg|left|200px]]<br /><applet load="1jxm" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1jxm, resolution 2.00Å" />

'''CRYSTAL STRUCTURE OF THE GMP BOUND SH3-HOOK-GK FRAGMENT OF PSD-95'''<br />

==Overview==

PSD-95/SAP90 is a member of the MAGUK superfamily. In excitatory synapses, PSD-95 clusters receptors and ion channels at specific sites in the, postsynaptic membrane and organizes downstream signaling and cytoskeletal, molecules. We have determined the crystal structures of the apo and, GMP-bound forms to 2.3 and 2.0 A resolutions, respectively, of a fragment, containing the SH3, HOOK, and guanylate kinase (GK) domains of PSD-95. We, observe an intramolecular interaction between the SH3 and GK domains, involving the formation of a beta sheet including residues N- and, C-terminal to the GK domain. Based on amino acid conservation and, mutational data available in the literature, we propose that this, intramolecular interaction is a common feature among MAGUK proteins.

PSD-95/SAP90 is a member of the MAGUK superfamily. In excitatory synapses, PSD-95 clusters receptors and ion channels at specific sites in the postsynaptic membrane and organizes downstream signaling and cytoskeletal molecules. We have determined the crystal structures of the apo and GMP-bound forms to 2.3 and 2.0 A resolutions, respectively, of a fragment containing the SH3, HOOK, and guanylate kinase (GK) domains of PSD-95. We observe an intramolecular interaction between the SH3 and GK domains involving the formation of a beta sheet including residues N- and C-terminal to the GK domain. Based on amino acid conservation and mutational data available in the literature, we propose that this intramolecular interaction is a common feature among MAGUK proteins.

==About this Structure==

1JXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with 5GP, GAI and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1JXM OCA].

1JXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=5GP:'>5GP</scene>, <scene name='pdbligand=GAI:'>GAI</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXM OCA].

==Reference==

Line 13:

[[Category: Rattus norvegicus]]

[[Category: Single protein]]

[[Category: Brunger, A.T.]]

[[Category: Brunger, A T.]]

[[Category: Panepucci, E.H.]]

[[Category: Panepucci, E H.]]

[[Category: Tavares, G.A.]]

[[Category: Tavares, G A.]]

[[Category: 5GP]]

[[Category: GAI]]

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[[Category: sh3 domain]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 18~~:40:~~09 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:49 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1jxm.jpg|left|200px]]<br /><applet load="1jxm" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jxm.jpg|left|200px]]<br /><applet load="1jxm" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jxm, resolution 2.00&Aring;" />
 '''CRYSTAL STRUCTURE OF THE GMP BOUND SH3-HOOK-GK FRAGMENT OF PSD-95'''<br />
 ==Overview==
-PSD-95/SAP90 is a member of the MAGUK superfamily. In excitatory synapses, PSD-95 clusters receptors and ion channels at specific sites in the, postsynaptic membrane and organizes downstream signaling and cytoskeletal, molecules. We have determined the crystal structures of the apo and, GMP-bound forms to 2.3 and 2.0 A resolutions, respectively, of a fragment, containing the SH3, HOOK, and guanylate kinase (GK) domains of PSD-95. We, observe an intramolecular interaction between the SH3 and GK domains, involving the formation of a beta sheet including residues N- and, C-terminal to the GK domain. Based on amino acid conservation and, mutational data available in the literature, we propose that this, intramolecular interaction is a common feature among MAGUK proteins.
+PSD-95/SAP90 is a member of the MAGUK superfamily. In excitatory synapses, PSD-95 clusters receptors and ion channels at specific sites in the postsynaptic membrane and organizes downstream signaling and cytoskeletal molecules. We have determined the crystal structures of the apo and GMP-bound forms to 2.3 and 2.0 A resolutions, respectively, of a fragment containing the SH3, HOOK, and guanylate kinase (GK) domains of PSD-95. We observe an intramolecular interaction between the SH3 and GK domains involving the formation of a beta sheet including residues N- and C-terminal to the GK domain. Based on amino acid conservation and mutational data available in the literature, we propose that this intramolecular interaction is a common feature among MAGUK proteins.
 ==About this Structure==
-JXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with 5GP, GAI and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JXM OCA].
+JXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=5GP:'>5GP</scene>, <scene name='pdbligand=GAI:'>GAI</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXM OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Rattus norvegicus]]
 [[Category: Single protein]]
-[[Category: Brunger, A.T.]]
+[[Category: Brunger, A T.]]
-[[Category: Panepucci, E.H.]]
+[[Category: Panepucci, E H.]]
-[[Category: Tavares, G.A.]]
+[[Category: Tavares, G A.]]
 [[Category: 5GP]]
 [[Category: GAI]]
@@ Line 24: / Line 24: @@
 [[Category: sh3 domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:40:09 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:49 2008''