1mwg: Difference between revisions
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==STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE== | |||
[[ | <StructureSection load='1mwg' size='340' side='right' caption='[[1mwg]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1mwg]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MWG FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mwg RCSB], [http://www.ebi.ac.uk/pdbsum/1mwg PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities. | |||
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach.,Wu M, Turner DH Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939<ref>PMID:8703939</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
[[Category: Santalucia, J.]] | [[Category: Santalucia, J.]] | ||
[[Category: Turner, D H.]] | [[Category: Turner, D H.]] | ||
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[[Category: G:a mismatch]] | [[Category: G:a mismatch]] | ||
[[Category: Ribonucleic acid]] | [[Category: Ribonucleic acid]] | ||
[[Category: Rna]] | |||
Revision as of 06:59, 9 June 2014
STRUCTURE OF RIBONUCLEIC ACID, NMR, MINIMIZED AVERAGE STRUCTURE
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