1k3f: Difference between revisions

[[Image:1k3f.jpg|left|200px]]<br /><applet load="1k3f" size="350" color="white" frame="true" align="right" spinBox="true"  

Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 A resolution. The coordinates of the trigonal crystal form were used as a starting model and the refinement by the program XPLOR led to the R-factor of 18.6%. The amino acid fold of the protein was found to be the same as that in the trigonal crystals. The positions of flexible regions were refined. The conclusion about the involvement in the active site is in good agreement with the results of the biochemical experiments.

1K3F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3F OCA].  

[[Category: Armstrong, S R.]]

[[Category: Blagova, E V.]]

[[Category: Burlakova, A A.]]

[[Category: Debabov, V G.]]

[[Category: Ealick, S E.]]

[[Category: Komissarov, A A.]]

[[Category: Linkova, E V.]]

[[Category: Mikhailov, A M.]]

[[Category: Mironov, A S.]]

[[Category: Morgunova, E Yu.]]

[[Category: Popov, A N.]]

[[Category: Smirnova, E A.]]

[[Category: Vainshtein, B K.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:39 2008''