1k3s: Difference between revisions
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New page: left|200px<br /><applet load="1k3s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k3s, resolution 1.9Å" /> '''Type III Secretion Ch... |
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[[Image:1k3s.jpg|left|200px]]<br /><applet load="1k3s" size=" | [[Image:1k3s.jpg|left|200px]]<br /><applet load="1k3s" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1k3s, resolution 1.9Å" /> | caption="1k3s, resolution 1.9Å" /> | ||
'''Type III Secretion Chaperone SigE'''<br /> | '''Type III Secretion Chaperone SigE'''<br /> | ||
==Overview== | ==Overview== | ||
Several Gram-negative bacterial pathogens have evolved a type III | Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones. | ||
==About this Structure== | ==About this Structure== | ||
1K3S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1K3S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3S OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Salmonella enterica]] | [[Category: Salmonella enterica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bertero, M | [[Category: Bertero, M G.]] | ||
[[Category: Creagh, L.]] | [[Category: Creagh, L.]] | ||
[[Category: Finlay, B | [[Category: Finlay, B B.]] | ||
[[Category: Frey, E | [[Category: Frey, E A.]] | ||
[[Category: Lim, D.]] | [[Category: Lim, D.]] | ||
[[Category: Luo, Y.]] | [[Category: Luo, Y.]] | ||
[[Category: Marcus, S | [[Category: Marcus, S L.]] | ||
[[Category: Pfuetzner, R | [[Category: Pfuetzner, R A.]] | ||
[[Category: Strynadka, N | [[Category: Strynadka, N C.J.]] | ||
[[Category: Wenk, M | [[Category: Wenk, M R.]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: chaperone]] | [[Category: chaperone]] | ||
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[[Category: type iii]] | [[Category: type iii]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:51 2008'' | ||
