1k64: Difference between revisions
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New page: left|200px<br /><applet load="1k64" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k64" /> '''NMR Structue of alpha-conotoxin EI'''<br /> ... |
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'''NMR Structue of alpha-conotoxin EI'''<br /> | '''NMR Structue of alpha-conotoxin EI'''<br /> | ||
==Overview== | ==Overview== | ||
A high resolution structure of alpha-conotoxin EI has been determined by | A high resolution structure of alpha-conotoxin EI has been determined by (1)H NMR spectroscopy and molecular modeling. alpha-Conotoxin EI has the same disulfide framework as alpha 4/7 conotoxins targeting neuronal nicotinic acetylcholine receptors but antagonizes the neuromuscular receptor as do the alpha 3/5 and alpha A conotoxins. The unique binding preference of alpha-conotoxin EI to the alpha(1)/delta subunit interface of Torpedo neuromuscular receptor makes it a valuable structural template for superposition of various alpha-conotoxins possessing distinct receptor subtype specificities. Structural comparison of alpha-conotoxin EI with the gamma-subunit favoring alpha-conotoxin GI suggests that the Torpedo delta-subunit preference of the former originates from its second loop. Superposition of three-dimensional structures of seven alpha-conotoxins reveals that the estimated size of the toxin-binding pocket in nicotinic acetylcholine receptor is approximately 20 A (height) x 20 A (width) x 15 A (thickness). | ||
==About this Structure== | ==About this Structure== | ||
1K64 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1K64 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K64 OCA]. | ||
==Reference== | ==Reference== | ||
Solution conformation of alpha-conotoxin EI, a neuromuscular toxin specific for the alpha 1/delta subunit interface of torpedo nicotinic acetylcholine receptor., Park KH, Suk JE, Jacobsen R, Gray WR, McIntosh JM, Han KH, J Biol Chem. 2001 Dec 28;276(52):49028-33. Epub 2001 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11641403 11641403] | Solution conformation of alpha-conotoxin EI, a neuromuscular toxin specific for the alpha 1/delta subunit interface of torpedo nicotinic acetylcholine receptor., Park KH, Suk JE, Jacobsen R, Gray WR, McIntosh JM, Han KH, J Biol Chem. 2001 Dec 28;276(52):49028-33. Epub 2001 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11641403 11641403] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gray, W | [[Category: Gray, W R.]] | ||
[[Category: Han, K | [[Category: Han, K H.]] | ||
[[Category: Jacobsen, R.]] | [[Category: Jacobsen, R.]] | ||
[[Category: McIntosh, J | [[Category: McIntosh, J M.]] | ||
[[Category: Park, K | [[Category: Park, K H.]] | ||
[[Category: Suk, J | [[Category: Suk, J E.]] | ||
[[Category: NH2]] | [[Category: NH2]] | ||
[[Category: omega-shaped containing a-helix]] | [[Category: omega-shaped containing a-helix]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:32 2008'' | ||
