1k6a: Difference between revisions

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[[Image:1k6a.gif|left|200px]]<br /><applet load="1k6a" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1k6a.gif|left|200px]]<br /><applet load="1k6a" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1k6a, resolution 1.14Å" />

'''Structural studies on the mobility in the active site of the Thermoascus aurantiacus xylanase I'''<br />

==Overview==

The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX), has been investigated both biochemically and structurally. High resolution, crystallographic analyses at 291 K and 100 K of TAX complexes with, xylobiose show that the ligand is in its alpha anomeric conformation and, provide a rationale for specificity on p-nitrophenyl glycosides at the -1, and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in, family 10 are identified, which differ by the presence or absence of a, short helical stretch in the eighth betaalpha-loop of the TIM barrel, the, loop bearing Trp 275. This structural difference is discussed in the, context of Trp 275 mobility and xylanase function.

The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX) has been investigated both biochemically and structurally. High resolution crystallographic analyses at 291 K and 100 K of TAX complexes with xylobiose show that the ligand is in its alpha anomeric conformation and provide a rationale for specificity on p-nitrophenyl glycosides at the -1 and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in family 10 are identified, which differ by the presence or absence of a short helical stretch in the eighth betaalpha-loop of the TIM barrel, the loop bearing Trp 275. This structural difference is discussed in the context of Trp 275 mobility and xylanase function.

==About this Structure==

1K6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. This structure ~~superseeds~~ the now removed PDB entry 1FXM. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1K6A OCA].

1K6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. This structure supersedes the now removed PDB entry 1FXM. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6A OCA].

==Reference==

Line 15:

[[Category: Thermoascus aurantiacus]]

[[Category: Andrei, C.]]

[[Category: Bhat, M.K.]]

[[Category: Bhat, M K.]]

[[Category: Eckert, K.]]

[[Category: Kalogiannis, S.]]

[[Category: Larsen, S.]]

[[Category: Leggio, L.Lo.]]

[[Category: Leggio, L Lo.]]

[[Category: Pickersgill, R.W.]]

[[Category: Pickersgill, R W.]]

[[Category: Teixeira, S.C.M.]]

[[Category: Teixeira, S C.M.]]

[[Category: active site mobility]]

[[Category: alternate conformations]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 18~~:53:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:35 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1k6a.gif|left|200px]]<br /><applet load="1k6a" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k6a.gif|left|200px]]<br /><applet load="1k6a" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1k6a, resolution 1.14&Aring;" />
 '''Structural studies on the mobility in the active site of the Thermoascus aurantiacus xylanase I'''<br />
 ==Overview==
-The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX), has been investigated both biochemically and structurally. High resolution, crystallographic analyses at 291 K and 100 K of TAX complexes with, xylobiose show that the ligand is in its alpha anomeric conformation and, provide a rationale for specificity on p-nitrophenyl glycosides at the -1, and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in, family 10 are identified, which differ by the presence or absence of a, short helical stretch in the eighth betaalpha-loop of the TIM barrel, the, loop bearing Trp 275. This structural difference is discussed in the, context of Trp 275 mobility and xylanase function.
+The substrate specificity of Thermoascus aurantiacus xylanase 10A (TAX) has been investigated both biochemically and structurally. High resolution crystallographic analyses at 291 K and 100 K of TAX complexes with xylobiose show that the ligand is in its alpha anomeric conformation and provide a rationale for specificity on p-nitrophenyl glycosides at the -1 and -2 subsites. Trp 275, which is disordered in uncomplexed structures, is stabilised by its interaction with xylobiose. Two structural subsets in family 10 are identified, which differ by the presence or absence of a short helical stretch in the eighth betaalpha-loop of the TIM barrel, the loop bearing Trp 275. This structural difference is discussed in the context of Trp 275 mobility and xylanase function.
 ==About this Structure==
-K6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. This structure superseeds the now removed PDB entry 1FXM. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K6A OCA].
+K6A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. This structure supersedes the now removed PDB entry 1FXM. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6A OCA].
 ==Reference==
@@ Line 15: / Line 15: @@
 [[Category: Thermoascus aurantiacus]]
 [[Category: Andrei, C.]]
-[[Category: Bhat, M.K.]]
+[[Category: Bhat, M K.]]
 [[Category: Eckert, K.]]
 [[Category: Kalogiannis, S.]]
 [[Category: Larsen, S.]]
-[[Category: Leggio, L.Lo.]]
+[[Category: Leggio, L Lo.]]
-[[Category: Pickersgill, R.W.]]
+[[Category: Pickersgill, R W.]]
-[[Category: Teixeira, S.C.M.]]
+[[Category: Teixeira, S C.M.]]
 [[Category: active site mobility]]
 [[Category: alternate conformations]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:53:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:35 2008''