1kdg: Difference between revisions

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[[Image:1kdg.gif|left|200px]]<br /><applet load="1kdg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1kdg.gif|left|200px]]<br /><applet load="1kdg" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1kdg, resolution 1.50Å" />

'''Crystal structure of the flavin domain of cellobiose dehydrogenase'''<br />

==Overview==

Cellobiose dehydrogenase (CDH) participates in the degradation of, cellulose and lignin. The protein is an extracellular flavocytochrome with, a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase, domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the, anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and, the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure, of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution., DH(cdh) belongs to the GMC family of oxidoreductases, which includes, glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence, identity with members of the family is low. The overall fold of DH(cdh) is, p-hydroxybenzoate hydroxylase-like and is similar to, but also different, from, that of GOX and COX. It is partitioned into an FAD-binding subdomain, of alpha/beta type and a substrate-binding subdomain consisting of a, seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh), suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and, that the resulting distance between the cofactors is within acceptable, limits for inter-domain electron transfer. Based on docking of the, substrate, cellobiose, in the active site of DH(cdh), we propose that the, enzyme discriminates against glucose by favouring interaction with the, non-reducing end of cellobiose.

Cellobiose dehydrogenase (CDH) participates in the degradation of cellulose and lignin. The protein is an extracellular flavocytochrome with a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution. DH(cdh) belongs to the GMC family of oxidoreductases, which includes glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence identity with members of the family is low. The overall fold of DH(cdh) is p-hydroxybenzoate hydroxylase-like and is similar to, but also different from, that of GOX and COX. It is partitioned into an FAD-binding subdomain of alpha/beta type and a substrate-binding subdomain consisting of a seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh) suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and that the resulting distance between the cofactors is within acceptable limits for inter-domain electron transfer. Based on docking of the substrate, cellobiose, in the active site of DH(cdh), we propose that the enzyme discriminates against glucose by favouring interaction with the non-reducing end of cellobiose.

==About this Structure==

1KDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with NAG, MAN, HG, 6FA, EMT and S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].

1KDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=6FA:'>6FA</scene>, <scene name='pdbligand=EMT:'>EMT</scene> and <scene name='pdbligand=S:'>S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Divne, C.]]

[[Category: Hallberg, B.M.]]

[[Category: Hallberg, B M.]]

[[Category: Henriksson, G.]]

[[Category: Pettersson, G.]]

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[[Category: rossmann fold]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:50 2008''

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-[[Image:1kdg.gif|left|200px]]<br /><applet load="1kdg" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kdg.gif|left|200px]]<br /><applet load="1kdg" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1kdg, resolution 1.50&Aring;" />
 '''Crystal structure of the flavin domain of cellobiose dehydrogenase'''<br />
 ==Overview==
-Cellobiose dehydrogenase (CDH) participates in the degradation of, cellulose and lignin. The protein is an extracellular flavocytochrome with, a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase, domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the, anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and, the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure, of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution., DH(cdh) belongs to the GMC family of oxidoreductases, which includes, glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence, identity with members of the family is low. The overall fold of DH(cdh) is, p-hydroxybenzoate hydroxylase-like and is similar to, but also different, from, that of GOX and COX. It is partitioned into an FAD-binding subdomain, of alpha/beta type and a substrate-binding subdomain consisting of a, seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh), suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and, that the resulting distance between the cofactors is within acceptable, limits for inter-domain electron transfer. Based on docking of the, substrate, cellobiose, in the active site of DH(cdh), we propose that the, enzyme discriminates against glucose by favouring interaction with the, non-reducing end of cellobiose.
+Cellobiose dehydrogenase (CDH) participates in the degradation of cellulose and lignin. The protein is an extracellular flavocytochrome with a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution. DH(cdh) belongs to the GMC family of oxidoreductases, which includes glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence identity with members of the family is low. The overall fold of DH(cdh) is p-hydroxybenzoate hydroxylase-like and is similar to, but also different from, that of GOX and COX. It is partitioned into an FAD-binding subdomain of alpha/beta type and a substrate-binding subdomain consisting of a seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh) suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and that the resulting distance between the cofactors is within acceptable limits for inter-domain electron transfer. Based on docking of the substrate, cellobiose, in the active site of DH(cdh), we propose that the enzyme discriminates against glucose by favouring interaction with the non-reducing end of cellobiose.
 ==About this Structure==
-KDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with NAG, MAN, HG, 6FA, EMT and S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].
+KDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=6FA:'>6FA</scene>, <scene name='pdbligand=EMT:'>EMT</scene> and <scene name='pdbligand=S:'>S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDG OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Divne, C.]]
-[[Category: Hallberg, B.M.]]
+[[Category: Hallberg, B M.]]
 [[Category: Henriksson, G.]]
 [[Category: Pettersson, G.]]
@@ Line 29: / Line 29: @@
 [[Category: rossmann fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:04:57 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:50 2008''