1kix: Difference between revisions
New page: left|200px<br /><applet load="1kix" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kix, resolution 2.70Å" /> '''Dimeric Structure of... |
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[[Image:1kix.gif|left|200px]]<br /><applet load="1kix" size=" | [[Image:1kix.gif|left|200px]]<br /><applet load="1kix" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kix, resolution 2.70Å" /> | caption="1kix, resolution 2.70Å" /> | ||
'''Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA'''<br /> | '''Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA'''<br /> | ||
==Overview== | ==Overview== | ||
Telomeres are the specialized protein--DNA complexes that cap and protect | Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures. | ||
==About this Structure== | ==About this Structure== | ||
1KIX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1KIX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIX OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sterkiella nova]] | [[Category: Sterkiella nova]] | ||
[[Category: Peersen, O | [[Category: Peersen, O B.]] | ||
[[Category: Ruggles, J | [[Category: Ruggles, J A.]] | ||
[[Category: Schultz, S | [[Category: Schultz, S C.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: dna-protein interactions]] | [[Category: dna-protein interactions]] | ||
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[[Category: telomere binding protein]] | [[Category: telomere binding protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:38 2008'' | ||
Revision as of 11:34, 21 February 2008
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Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA
Overview
Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures.
About this Structure
1KIX is a Single protein structure of sequence from Sterkiella nova with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA., Peersen OB, Ruggles JA, Schultz SC, Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536
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