1kk0: Difference between revisions

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[[Image:1kk0.gif|left|200px]]<br /><applet load="1kk0" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1kk0.gif|left|200px]]<br /><applet load="1kk0" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1kk0, resolution 1.95Å" />

'''Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi'''<br />

==Overview==

The heterotrimeric factor e/aIF2 plays a central role in, eukaryotic/archaeal initiation of translation. By delivering the initiator, methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation, codon selection. The three subunits of aIF2 from the hyperthermophilic, archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The, beta and gamma subunits each contain a tightly bound zinc. The large gamma, subunit is shown to form the structural core for trimer assembly. The, crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or, GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma, displays marked similarities to elongation factors. A distinctive feature, of e/aIF2gamma is a subdomain containing a zinc-binding knuckle., Examination of the nucleotide-complexed aIF2gamma structures suggests, mechanisms of action and tRNA binding properties similar to those of an, elongation factor. Implications for the mechanism of translation, initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the, search for the initiation codon is envisaged.

The heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal initiation of translation. By delivering the initiator methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation codon selection. The three subunits of aIF2 from the hyperthermophilic archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The beta and gamma subunits each contain a tightly bound zinc. The large gamma subunit is shown to form the structural core for trimer assembly. The crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma displays marked similarities to elongation factors. A distinctive feature of e/aIF2gamma is a subdomain containing a zinc-binding knuckle. Examination of the nucleotide-complexed aIF2gamma structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor. Implications for the mechanism of translation initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the search for the initiation codon is envisaged.

==About this Structure==

1KK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1KK0 OCA].

1KK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK0 OCA].

==Reference==

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[[Category: initiation of translation]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 19~~:18:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:58 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1kk0.gif|left|200px]]<br /><applet load="1kk0" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kk0.gif|left|200px]]<br /><applet load="1kk0" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1kk0, resolution 1.95&Aring;" />
 '''Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi'''<br />
 ==Overview==
-The heterotrimeric factor e/aIF2 plays a central role in, eukaryotic/archaeal initiation of translation. By delivering the initiator, methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation, codon selection. The three subunits of aIF2 from the hyperthermophilic, archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The, beta and gamma subunits each contain a tightly bound zinc. The large gamma, subunit is shown to form the structural core for trimer assembly. The, crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or, GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma, displays marked similarities to elongation factors. A distinctive feature, of e/aIF2gamma is a subdomain containing a zinc-binding knuckle., Examination of the nucleotide-complexed aIF2gamma structures suggests, mechanisms of action and tRNA binding properties similar to those of an, elongation factor. Implications for the mechanism of translation, initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the, search for the initiation codon is envisaged.
+The heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal initiation of translation. By delivering the initiator methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation codon selection. The three subunits of aIF2 from the hyperthermophilic archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The beta and gamma subunits each contain a tightly bound zinc. The large gamma subunit is shown to form the structural core for trimer assembly. The crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma displays marked similarities to elongation factors. A distinctive feature of e/aIF2gamma is a subdomain containing a zinc-binding knuckle. Examination of the nucleotide-complexed aIF2gamma structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor. Implications for the mechanism of translation initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the search for the initiation codon is envisaged.
 ==About this Structure==
-KK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KK0 OCA].
+KK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK0 OCA].
 ==Reference==
@@ Line 19: / Line 19: @@
 [[Category: initiation of translation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:18:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:58 2008''