1kkh: Difference between revisions
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New page: left|200px<br /><applet load="1kkh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kkh, resolution 2.40Å" /> '''Crystal Structure of... |
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[[Image:1kkh.gif|left|200px]]<br /><applet load="1kkh" size=" | [[Image:1kkh.gif|left|200px]]<br /><applet load="1kkh" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kkh, resolution 2.40Å" /> | caption="1kkh, resolution 2.40Å" /> | ||
'''Crystal Structure of the Methanococcus jannaschii Mevalonate Kinase'''<br /> | '''Crystal Structure of the Methanococcus jannaschii Mevalonate Kinase'''<br /> | ||
==Overview== | ==Overview== | ||
The mevalonate-dependent pathway is used by many organisms to synthesize | The mevalonate-dependent pathway is used by many organisms to synthesize isopentenyl pyrophosphate, the building block for the biosynthesis of many biologically important compounds, including farnesyl pyrophosphate, dolichol, and many sterols. Mevalonate kinase (MVK) catalyzes a critical phosphoryl transfer step, producing mevalonate 5'-phosphate. The crystal structure of thermostable MVK from Methanococcus jannaschii has been determined at 2.4 A, revealing an overall fold similar to the homoserine kinase from M. jannaschii. In addition, the enzyme shows structural similarity with mevalonate 5-diphosphate decarboxylase and domain IV of elongation factor G. The active site of MVK is in the cleft between its N- and C-terminal domains. Several structural motifs conserved among species, including a phosphate-binding loop, have been found in this cavity. Asp(155), an invariant residue among MVK sequences, is located close to the putative phosphate-binding site and has been assumed to play the catalytic role. Analysis of the MVK model in the context of the other members of the GHMP kinase family offers the opportunity to understand both the mechanism of these enzymes and the structural details that may lead to the design of novel drugs. | ||
==About this Structure== | ==About this Structure== | ||
1KKH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with DIO as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Mevalonate_kinase Mevalonate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.36 2.7.1.36] Full crystallographic information is available from [http:// | 1KKH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=DIO:'>DIO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Mevalonate_kinase Mevalonate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.36 2.7.1.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKH OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mevalonate kinase]] | [[Category: Mevalonate kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Roessner, C | [[Category: Roessner, C A.]] | ||
[[Category: Sacchettini, J | [[Category: Sacchettini, J C.]] | ||
[[Category: Scott, A | [[Category: Scott, A I.]] | ||
[[Category: Shipman, L | [[Category: Shipman, L W.]] | ||
[[Category: Yang, D.]] | [[Category: Yang, D.]] | ||
[[Category: DIO]] | [[Category: DIO]] | ||
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[[Category: phosphate-binding loop]] | [[Category: phosphate-binding loop]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:35:08 2008'' | ||
