5msf: Difference between revisions

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[[Image:5msf.gif|left|200px]]<br /><applet load="5msf" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:5msf.gif|left|200px]]<br /><applet load="5msf" size="350" color="white" frame="true" align="right" spinBox="true"

caption="5msf, resolution 2.80Å" />

'''MS2 PROTEIN CAPSID/RNA COMPLEX'''<br />

==Overview==

We have determined the crystal structures, at 2.8 A resolution, of two, different RNA aptamers, each bound to MS2 coat protein. One of the, aptamers contains a non-Watson-Crick base pair, while the other is missing, one of the unpaired adenines that make sequence-specific contacts in the, wild-type complex. Despite these differences, the RNA aptamers bind in the, same location on the protein as the wild-type translational operator., Comparison of these new structures with other MS2-RNA complexes allows us, to refine further the definition of the minimal recognition elements and, suggests a possible application of the MS2 system for routine structure, determination of small nucleic acid motifs.

We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.

==About this Structure==

5MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=5MSF OCA].

5MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSF OCA].

==Reference==

Line 13:

[[Category: Enterobacterio phage ms2]]

[[Category: Single protein]]

[[Category: Adams, C.J.]]

[[Category: Adams, C J.]]

[[Category: Convery, M.A.]]

[[Category: Convery, M A.]]

[[Category: Ellington, A.D.]]

[[Category: Ellington, A D.]]

[[Category: Hirao, I.]]

[[Category: Peabody, D.S.]]

[[Category: Peabody, D S.]]

[[Category: Phillips, S.E.V.]]

[[Category: Phillips, S E.V.]]

[[Category: Rowsell, S.]]

[[Category: Stockley, P.G.]]

[[Category: Stockley, P G.]]

[[Category: Stonehouse, N.J.]]

[[Category: Stonehouse, N J.]]

[[Category: bacteriophage ms2]]

[[Category: capsid protein ms2/rna aptamer complex]]

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[[Category: rna-protein complex]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:15:31 2008''

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@@ Line 1: / Line 1: @@
-[[Image:5msf.gif|left|200px]]<br /><applet load="5msf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:5msf.gif|left|200px]]<br /><applet load="5msf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="5msf, resolution 2.80&Aring;" />
 '''MS2 PROTEIN CAPSID/RNA COMPLEX'''<br />
 ==Overview==
-We have determined the crystal structures, at 2.8 A resolution, of two, different RNA aptamers, each bound to MS2 coat protein. One of the, aptamers contains a non-Watson-Crick base pair, while the other is missing, one of the unpaired adenines that make sequence-specific contacts in the, wild-type complex. Despite these differences, the RNA aptamers bind in the, same location on the protein as the wild-type translational operator., Comparison of these new structures with other MS2-RNA complexes allows us, to refine further the definition of the minimal recognition elements and, suggests a possible application of the MS2 system for routine structure, determination of small nucleic acid motifs.
+We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.
 ==About this Structure==
-MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5MSF OCA].
+MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSF OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Enterobacterio phage ms2]]
 [[Category: Single protein]]
-[[Category: Adams, C.J.]]
+[[Category: Adams, C J.]]
-[[Category: Convery, M.A.]]
+[[Category: Convery, M A.]]
-[[Category: Ellington, A.D.]]
+[[Category: Ellington, A D.]]
 [[Category: Hirao, I.]]
-[[Category: Peabody, D.S.]]
+[[Category: Peabody, D S.]]
-[[Category: Phillips, S.E.V.]]
+[[Category: Phillips, S E.V.]]
 [[Category: Rowsell, S.]]
-[[Category: Stockley, P.G.]]
+[[Category: Stockley, P G.]]
-[[Category: Stonehouse, N.J.]]
+[[Category: Stonehouse, N J.]]
 [[Category: bacteriophage ms2]]
 [[Category: capsid protein ms2/rna aptamer complex]]
@@ Line 28: / Line 28: @@
 [[Category: rna-protein complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:37:15 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:15:31 2008''