4rnp: Difference between revisions

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[[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="4rnp, resolution 3.0Å" />

'''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''<br />

==Overview==

The crystal structure of T7 RNA polymerase reveals a molecule organized, around a cleft that can accommodate a double-stranded DNA template. A, portion (approximately 45%) of the molecule displays extensive structural, homology to the polymerase domain of Klenow fragment and more limited, homology to the human immunodeficiency virus HIV-1 reverse transcriptase., A comparison of the structures and sequences of these polymerases, identifies structural elements that may be responsible for discriminating, between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA, templates. The relative locations of the catalytic site and a specific, promoter recognition residue allow the orientation of the polymerase on, the template to be defined.

The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.

==About this Structure==

4RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure ~~superseeds~~ the now removed PDB entries 3RNP and 2RNP. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA].

4RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure supersedes the now removed PDB entries 3RNP and 2RNP. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA].

==Reference==

Line 14:

[[Category: DNA-directed RNA polymerase]]

[[Category: Single protein]]

[[Category: Liu, Z.J.]]

[[Category: Liu, Z J.]]

[[Category: Rose, J.P.]]

[[Category: Rose, J P.]]

[[Category: Sousa, R.]]

[[Category: Wang, B.C.]]

[[Category: Wang, B C.]]

[[Category: nucleotidyltransferase]]

[[Category: rna polymerase]]

[[Category: transcription]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 19:47:~~11 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:14:15 2008''

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@@ Line 1: / Line 1: @@
-[[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:4rnp.jpg|left|200px]]<br /><applet load="4rnp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="4rnp, resolution 3.0&Aring;" />
 '''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''<br />
 ==Overview==
-The crystal structure of T7 RNA polymerase reveals a molecule organized, around a cleft that can accommodate a double-stranded DNA template. A, portion (approximately 45%) of the molecule displays extensive structural, homology to the polymerase domain of Klenow fragment and more limited, homology to the human immunodeficiency virus HIV-1 reverse transcriptase., A comparison of the structures and sequences of these polymerases, identifies structural elements that may be responsible for discriminating, between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA, templates. The relative locations of the catalytic site and a specific, promoter recognition residue allow the orientation of the polymerase on, the template to be defined.
+The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.
 ==About this Structure==
-RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure superseeds the now removed PDB entries 3RNP and 2RNP. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA].
+RNP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. This structure supersedes the now removed PDB entries 3RNP and 2RNP. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: DNA-directed RNA polymerase]]
 [[Category: Single protein]]
-[[Category: Liu, Z.J.]]
+[[Category: Liu, Z J.]]
-[[Category: Rose, J.P.]]
+[[Category: Rose, J P.]]
 [[Category: Sousa, R.]]
-[[Category: Wang, B.C.]]
+[[Category: Wang, B C.]]
 [[Category: nucleotidyltransferase]]
 [[Category: rna polymerase]]
 [[Category: transcription]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:47:11 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:14:15 2008''