1kuj: Difference between revisions

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[[Image:1kuj.gif|left|200px]]<br /><applet load="1kuj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1kuj.gif|left|200px]]<br /><applet load="1kuj" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1kuj, resolution 2.00Å" />

'''Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose'''<br />

==Overview==

Evidence is presented that the specificity of jacalin, the seed lectin, from jack fruit (Artocarpus integrifolia), is not directed exclusively, against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and, galactose, but also against mannose and oligomannosides. Biochemical, analyses based on surface-plasmon-resonance measurements, combined with, the X-ray-crystallographic determination of the structure of a, jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated, clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and, N-acetylmuramic acid. Structural analyses demonstrated that the relatively, large size of the carbohydrate-binding site enables jacalin to accommodate, monosaccharides with different hydroxyl conformations and provided, unambiguous evidence that the beta-prism structure of jacalin is a, sufficiently flexible structural scaffold to confer different, carbohydrate-binding specificities to a single lectin.

Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.

==About this Structure==

1KUJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer] with MMA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA].

1KUJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer] with <scene name='pdbligand=MMA:'>MMA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA].

==Reference==

Line 13:

[[Category: Artocarpus integer]]

[[Category: Protein complex]]

[[Category: Astoul, C.H.]]

[[Category: Astoul, C H.]]

[[Category: Barre, A.]]

[[Category: Bourne, Y.]]

[[Category: Damme, E.J.M.Van.]]

[[Category: Damme, E J.M Van.]]

[[Category: Menu-Bouaouiche, L.]]

[[Category: Peumans, W.J.]]

[[Category: Peumans, W J.]]

[[Category: Rouge, P.]]

[[Category: Zamboni, V.]]

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[[Category: lectin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 19~~:53:~~20 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:38:01 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1kuj.gif|left|200px]]<br /><applet load="1kuj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kuj.gif|left|200px]]<br /><applet load="1kuj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1kuj, resolution 2.00&Aring;" />
 '''Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose'''<br />
 ==Overview==
-Evidence is presented that the specificity of jacalin, the seed lectin, from jack fruit (Artocarpus integrifolia), is not directed exclusively, against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and, galactose, but also against mannose and oligomannosides. Biochemical, analyses based on surface-plasmon-resonance measurements, combined with, the X-ray-crystallographic determination of the structure of a, jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated, clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and, N-acetylmuramic acid. Structural analyses demonstrated that the relatively, large size of the carbohydrate-binding site enables jacalin to accommodate, monosaccharides with different hydroxyl conformations and provided, unambiguous evidence that the beta-prism structure of jacalin is a, sufficiently flexible structural scaffold to confer different, carbohydrate-binding specificities to a single lectin.
+Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.
 ==About this Structure==
-KUJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer] with MMA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA].
+KUJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer] with <scene name='pdbligand=MMA:'>MMA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUJ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Artocarpus integer]]
 [[Category: Protein complex]]
-[[Category: Astoul, C.H.]]
+[[Category: Astoul, C H.]]
 [[Category: Barre, A.]]
 [[Category: Bourne, Y.]]
-[[Category: Damme, E.J.M.Van.]]
+[[Category: Damme, E J.M Van.]]
 [[Category: Menu-Bouaouiche, L.]]
-[[Category: Peumans, W.J.]]
+[[Category: Peumans, W J.]]
 [[Category: Rouge, P.]]
 [[Category: Zamboni, V.]]
@@ Line 26: / Line 26: @@
 [[Category: lectin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:53:20 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:38:01 2008''