3sxl: Difference between revisions

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[[Image:3sxl.gif|left|200px]]<br /><applet load="3sxl" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:3sxl.gif|left|200px]]<br /><applet load="3sxl" size="350" color="white" frame="true" align="right" spinBox="true"

caption="3sxl, resolution 2.700Å" />

'''SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER'''<br />

==Overview==

By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs, sexual differentiation and dosage compensation in Drosophila melanogaster., To investigate the basis for RNA binding specificity, we determined the, crystal structure of the tandem RNA recognition motifs (RRMs) of SXL. Both, RRMs adopt the canonical RRM fold, and the 10-residue, interdomain linker, shows significant disorder. In contrast to the previously determined, structure of the two-RRM fragment of heterogeneous nuclear, ribonucleoprotein Al, SXL displays no interdomain contacts between RRMs., These results suggest that the SXL RRMs are flexibly tethered in solution, and RNA binding restricts the orientation of RRMs. Therefore, the observed, specificity for single-stranded, U-rich sequences does not arise from a, predefined, rigid architecture of the isolated SXL RRMs.

By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs sexual differentiation and dosage compensation in Drosophila melanogaster. To investigate the basis for RNA binding specificity, we determined the crystal structure of the tandem RNA recognition motifs (RRMs) of SXL. Both RRMs adopt the canonical RRM fold, and the 10-residue, interdomain linker shows significant disorder. In contrast to the previously determined structure of the two-RRM fragment of heterogeneous nuclear ribonucleoprotein Al, SXL displays no interdomain contacts between RRMs. These results suggest that the SXL RRMs are flexibly tethered in solution, and RNA binding restricts the orientation of RRMs. Therefore, the observed specificity for single-stranded, U-rich sequences does not arise from a predefined, rigid architecture of the isolated SXL RRMs.

==About this Structure==

3SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=3SXL OCA].

3SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXL OCA].

==Reference==

Line 13:

[[Category: Drosophila melanogaster]]

[[Category: Single protein]]

[[Category: Alber, T.C.]]

[[Category: Alber, T C.]]

[[Category: Crowder, S.M.]]

[[Category: Crowder, S M.]]

[[Category: Kanaar, R.]]

[[Category: Rio, D.C.]]

[[Category: Rio, D C.]]

[[Category: rbd]]

[[Category: rna binding domain]]

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[[Category: x chromosome dosage compensation]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 19:59:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:25 2008''

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@@ Line 1: / Line 1: @@
-[[Image:3sxl.gif|left|200px]]<br /><applet load="3sxl" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:3sxl.gif|left|200px]]<br /><applet load="3sxl" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="3sxl, resolution 2.700&Aring;" />
 '''SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER'''<br />
 ==Overview==
-By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs, sexual differentiation and dosage compensation in Drosophila melanogaster., To investigate the basis for RNA binding specificity, we determined the, crystal structure of the tandem RNA recognition motifs (RRMs) of SXL. Both, RRMs adopt the canonical RRM fold, and the 10-residue, interdomain linker, shows significant disorder. In contrast to the previously determined, structure of the two-RRM fragment of heterogeneous nuclear, ribonucleoprotein Al, SXL displays no interdomain contacts between RRMs., These results suggest that the SXL RRMs are flexibly tethered in solution, and RNA binding restricts the orientation of RRMs. Therefore, the observed, specificity for single-stranded, U-rich sequences does not arise from a, predefined, rigid architecture of the isolated SXL RRMs.
+By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs sexual differentiation and dosage compensation in Drosophila melanogaster. To investigate the basis for RNA binding specificity, we determined the crystal structure of the tandem RNA recognition motifs (RRMs) of SXL. Both RRMs adopt the canonical RRM fold, and the 10-residue, interdomain linker shows significant disorder. In contrast to the previously determined structure of the two-RRM fragment of heterogeneous nuclear ribonucleoprotein Al, SXL displays no interdomain contacts between RRMs. These results suggest that the SXL RRMs are flexibly tethered in solution, and RNA binding restricts the orientation of RRMs. Therefore, the observed specificity for single-stranded, U-rich sequences does not arise from a predefined, rigid architecture of the isolated SXL RRMs.
 ==About this Structure==
-SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3SXL OCA].
+SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXL OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Drosophila melanogaster]]
 [[Category: Single protein]]
-[[Category: Alber, T.C.]]
+[[Category: Alber, T C.]]
-[[Category: Crowder, S.M.]]
+[[Category: Crowder, S M.]]
 [[Category: Kanaar, R.]]
-[[Category: Rio, D.C.]]
+[[Category: Rio, D C.]]
 [[Category: rbd]]
 [[Category: rna binding domain]]
@@ Line 26: / Line 26: @@
 [[Category: x chromosome dosage compensation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:59:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:25 2008''