1lcs: Difference between revisions
New page: left|200px<br /><applet load="1lcs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lcs, resolution 2.5Å" /> '''RECEPTOR-BINDING DOMA... |
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[[Image:1lcs.gif|left|200px]]<br /><applet load="1lcs" size=" | [[Image:1lcs.gif|left|200px]]<br /><applet load="1lcs" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1lcs, resolution 2.5Å" /> | caption="1lcs, resolution 2.5Å" /> | ||
'''RECEPTOR-BINDING DOMAIN FROM SUBGROUP B FELINE LEUKEMIA VIRUS'''<br /> | '''RECEPTOR-BINDING DOMAIN FROM SUBGROUP B FELINE LEUKEMIA VIRUS'''<br /> | ||
==Overview== | ==Overview== | ||
Infection of T lymphocytes by the cytopathic retrovirus feline leukemia | Infection of T lymphocytes by the cytopathic retrovirus feline leukemia virus subgroup T (FeLV-T) requires FeLIX, a cellular coreceptor that is encoded by an endogenous provirus and closely resembles the receptor-binding domain (RBD) of feline leukemia virus subgroup B (FeLV-B). We determined the structure of FeLV-B RBD, which has FeLIX activity, to a 2.5-A resolution by X-ray crystallography. The structure of the receptor-specific subdomain of this glycoprotein differs dramatically from that of Friend murine leukemia virus (Fr-MLV), which binds a different cell surface receptor. Remarkably, we find that Fr-MLV RBD also activates FeLV-T infection of cells expressing the Fr-MLV receptor and that FeLV-B RBD is a competitive inhibitor of infection under these conditions. These studies suggest that FeLV-T infection relies on the following property of mammalian leukemia virus RBDs: the ability to couple interaction with one of a variety of receptors to the activation of a conserved membrane fusion mechanism. A comparison of the FeLV-B and Fr-MLV RBD structures illustrates how receptor-specific regions are linked to conserved elements critical for postbinding events in virus entry. | ||
==About this Structure== | ==About this Structure== | ||
1LCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses] with TOE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1LCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses] with <scene name='pdbligand=TOE:'>TOE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCS OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Viruses]] | [[Category: Viruses]] | ||
[[Category: Barnett, A | [[Category: Barnett, A L.]] | ||
[[Category: Cunningham, J | [[Category: Cunningham, J M.]] | ||
[[Category: Fass, D.]] | [[Category: Fass, D.]] | ||
[[Category: Li, W.]] | [[Category: Li, W.]] | ||
[[Category: Wensel, D | [[Category: Wensel, D L.]] | ||
[[Category: TOE]] | [[Category: TOE]] | ||
[[Category: antiparallel beta-sandwich glycoprotein]] | [[Category: antiparallel beta-sandwich glycoprotein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:46 2008'' | ||
Revision as of 11:43, 21 February 2008
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RECEPTOR-BINDING DOMAIN FROM SUBGROUP B FELINE LEUKEMIA VIRUS
Overview
Infection of T lymphocytes by the cytopathic retrovirus feline leukemia virus subgroup T (FeLV-T) requires FeLIX, a cellular coreceptor that is encoded by an endogenous provirus and closely resembles the receptor-binding domain (RBD) of feline leukemia virus subgroup B (FeLV-B). We determined the structure of FeLV-B RBD, which has FeLIX activity, to a 2.5-A resolution by X-ray crystallography. The structure of the receptor-specific subdomain of this glycoprotein differs dramatically from that of Friend murine leukemia virus (Fr-MLV), which binds a different cell surface receptor. Remarkably, we find that Fr-MLV RBD also activates FeLV-T infection of cells expressing the Fr-MLV receptor and that FeLV-B RBD is a competitive inhibitor of infection under these conditions. These studies suggest that FeLV-T infection relies on the following property of mammalian leukemia virus RBDs: the ability to couple interaction with one of a variety of receptors to the activation of a conserved membrane fusion mechanism. A comparison of the FeLV-B and Fr-MLV RBD structures illustrates how receptor-specific regions are linked to conserved elements critical for postbinding events in virus entry.
About this Structure
1LCS is a Single protein structure of sequence from Viruses with TOE as ligand. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of a coreceptor for infection by a pathogenic feline retrovirus., Barnett AL, Wensel DL, Li W, Fass D, Cunningham JM, J Virol. 2003 Feb;77(4):2717-29. PMID:12552012
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