1lju: Difference between revisions
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New page: left|200px<br /><applet load="1lju" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lju, resolution 1.4Å" /> '''X-RAY STRUCTURE OF C1... |
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[[Image:1lju.jpg|left|200px]]<br /><applet load="1lju" size=" | [[Image:1lju.jpg|left|200px]]<br /><applet load="1lju" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1lju, resolution 1.4Å" /> | caption="1lju, resolution 1.4Å" /> | ||
'''X-RAY STRUCTURE OF C15A ARSENATE REDUCTASE FROM PI258 COMPLEXED WITH ARSENITE'''<br /> | '''X-RAY STRUCTURE OF C15A ARSENATE REDUCTASE FROM PI258 COMPLEXED WITH ARSENITE'''<br /> | ||
==Overview== | ==Overview== | ||
The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate | The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate reduction, involving its P-loop structural motif and three redox active cysteines, has been unraveled. All essential intermediates are visualized with x-ray crystallography, and NMR is used to map dynamic regions in a key disulfide intermediate. Steady-state kinetics of ArsC mutants gives a view of the crucial residues for catalysis. ArsC combines a phosphatase-like nucleophilic displacement reaction with a unique intramolecular disulfide bond cascade. Within this cascade, the formation of a disulfide bond triggers a reversible "conformational switch" that transfers the oxidative equivalents to the surface of the protein, while releasing the reduced substrate. | ||
==About this Structure== | ==About this Structure== | ||
1LJU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] Full crystallographic information is available from [http:// | 1LJU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJU OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Martins, J | [[Category: Martins, J C.]] | ||
[[Category: Messens, J.]] | [[Category: Messens, J.]] | ||
[[Category: Willem, R.]] | [[Category: Willem, R.]] | ||
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[[Category: ptpase i fold]] | [[Category: ptpase i fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:33 2008'' | ||
