1lk0: Difference between revisions
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New page: left|200px<br /><applet load="1lk0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lk0, resolution 1.60Å" /> '''Disulfide intermedia... |
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[[Image:1lk0.jpg|left|200px]]<br /><applet load="1lk0" size=" | [[Image:1lk0.jpg|left|200px]]<br /><applet load="1lk0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1lk0, resolution 1.60Å" /> | caption="1lk0, resolution 1.60Å" /> | ||
'''Disulfide intermediate of C89L Arsenate reductase from pI258'''<br /> | '''Disulfide intermediate of C89L Arsenate reductase from pI258'''<br /> | ||
==Overview== | ==Overview== | ||
The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate | The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate reduction, involving its P-loop structural motif and three redox active cysteines, has been unraveled. All essential intermediates are visualized with x-ray crystallography, and NMR is used to map dynamic regions in a key disulfide intermediate. Steady-state kinetics of ArsC mutants gives a view of the crucial residues for catalysis. ArsC combines a phosphatase-like nucleophilic displacement reaction with a unique intramolecular disulfide bond cascade. Within this cascade, the formation of a disulfide bond triggers a reversible "conformational switch" that transfers the oxidative equivalents to the surface of the protein, while releasing the reduced substrate. | ||
==About this Structure== | ==About this Structure== | ||
1LK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with K and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] Full crystallographic information is available from [http:// | 1LK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=K:'>K</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arsenate_reductase_(glutaredoxin) Arsenate reductase (glutaredoxin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.4.1 1.20.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LK0 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
[[Category: Belle, K | [[Category: Belle, K Van.]] | ||
[[Category: Brosens, E.]] | [[Category: Brosens, E.]] | ||
[[Category: Desmyter, A.]] | [[Category: Desmyter, A.]] | ||
[[Category: Gieter, M | [[Category: Gieter, M De.]] | ||
[[Category: Martins, J | [[Category: Martins, J C.]] | ||
[[Category: Messens, J.]] | [[Category: Messens, J.]] | ||
[[Category: Wieruszeski, J | [[Category: Wieruszeski, J M.]] | ||
[[Category: Willem, R.]] | [[Category: Willem, R.]] | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
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[[Category: ptpase i fold]] | [[Category: ptpase i fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:39 2008'' | ||
